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Femia, Angelo Pietro; Luceri, Cristina; Lodovici, Maura; Crucitta, Stefania; Caderni, Giovanna

Gene Expression Profile of Colon Mucosa after Cytotoxic Insult in wt and Apc-Mutated Pirc Rats: Possible Relation to Resistance to Apoptosis during Carcinogenesis

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  • Media type: E-Article
  • Title: Gene Expression Profile of Colon Mucosa after Cytotoxic Insult in wt and Apc-Mutated Pirc Rats: Possible Relation to Resistance to Apoptosis during Carcinogenesis
  • Contributor: Femia, Angelo Pietro; Luceri, Cristina; Lodovici, Maura; Crucitta, Stefania; Caderni, Giovanna
  • imprint: Hindawi Limited, 2016
  • Published in: BioMed Research International
  • Language: English
  • DOI: 10.1155/2016/1310342
  • ISSN: 2314-6133; 2314-6141
  • Keywords: General Immunology and Microbiology ; General Biochemistry, Genetics and Molecular Biology ; General Medicine
  • Origination:
  • Footnote:
  • Description: <jats:p><jats:italic>Apc</jats:italic>-mutated Pirc rats, spontaneously developing intestinal tumours, are resistant to 1,2-dimethylhydrazine- (DMH-) induced colon apoptosis. To understand this phenomenon, we analyzed the expression of genotoxic stress-related genes<jats:italic> Mgmt</jats:italic>,<jats:italic> Gsta1</jats:italic>, and<jats:italic> Gstp1</jats:italic> in the colon of wt and Pirc rats in basal conditions and 24 h after DMH; plasmatic oxidant/antioxidant status was also evaluated. After DMH,<jats:italic> Mgmt</jats:italic> expression was increased in both genotypes but significantly only in wt rats;<jats:italic> Gsta1</jats:italic> expression was significantly increased in both genotypes.<jats:italic> Gstp1</jats:italic> expression did not vary after DMH but was lower in Pirc rats. Moreover, for each genotype, we studied by microarray technique whole gene expression profile after DMH. By unsupervised cluster analysis, 28 genes were differentially modulated between the two genotypes. Among them were interferon-induced genes<jats:italic> Irf7</jats:italic>,<jats:italic> Oas1a</jats:italic>,<jats:italic> Oasl2</jats:italic>, and<jats:italic> Isg15</jats:italic> and the transcription factor<jats:italic> Taf6l</jats:italic>, overexpressed in DMH-treated wt rats and unchanged in Pirc rats. RT-PCR confirmed their overexpression in DMH-treated wt rats and showed a slighter variation in DMH-treated Pirc rats. Taken together, despite a blunted induction of<jats:italic> Irf7</jats:italic>,<jats:italic> Oas1a</jats:italic>, and<jats:italic> Mgmt</jats:italic>, defective apoptosis in Pirc rats 24 h after DMH is not mirrored by major differences in gene expression compared with wt rats.</jats:p>
  • Access State: Open Access