Martínez-Hernández, Sandra L.;
Becerra-González, Viridiana M.;
Muñoz-Ortega, Martín H.;
Loera-Muro, Víctor M.;
Ávila-Blanco, Manuel E.;
Medina-Rosales, Marina N.;
Ventura-Juárez, Javier
Evaluation of the PEΔIII-LC3-KDEL3 Chimeric Protein of Entamoeba histolytica-Lectin as a Vaccine Candidate against Amebic Liver Abscess
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Media type:
E-Article
Title:
Evaluation of the PEΔIII-LC3-KDEL3 Chimeric Protein of Entamoeba histolytica-Lectin as a Vaccine Candidate against Amebic Liver Abscess
Contributor:
Martínez-Hernández, Sandra L.;
Becerra-González, Viridiana M.;
Muñoz-Ortega, Martín H.;
Loera-Muro, Víctor M.;
Ávila-Blanco, Manuel E.;
Medina-Rosales, Marina N.;
Ventura-Juárez, Javier
Published:
Hindawi Limited, 2021
Published in:
Journal of Immunology Research, 2021 (2021), Seite 1-12
Language:
English
DOI:
10.1155/2021/6697900
ISSN:
2314-7156;
2314-8861
Origination:
Footnote:
Description:
Entamoeba histolytica is an intestinal parasite that causes dysentery and amebic liver abscess. E. histolytica has the capability to invade host tissue by union of virulence factor Gal/GalNAc lectin; this molecule induces an adherence-inhibitory antibody response as well as to protect against amebic liver abscess (ALA). The present work showed the effect of the immunization with PEΔIII-LC3-KDEL3 recombinant protein. In vitro, this candidate vaccine inhibited adherence of E. histolytica trophozoites to HepG2 cell monolayer, avoiding the cytolysis, and in a hamster model, we observed a vaccine-induced protection against the damage to tissue liver and the inhibition of uncontrolled inflammation. PEΔIII-LC3-KDEL3 reduced the expression of TNF-α, IL-1β, and NF-κB in all immunized groups at 4- and 7-day postinfection. The levels of IL-10, FOXP3, and IFN-γ were elevated at 7 days. The immunohistochemistry assay confirmed this result, revealing an elevated quantity of +IFN-γ cells in the liver tissue. ALA formation in hamsters immunized was minimal, and few trophozoites were identified. Hence, immunization with PEΔIII-LC3-KDEL3 herein prevented invasive amebiasis, avoided an acute proinflammatory response, and activated a protective response within a short time. Finally, this recombinant protein induced an increase of serum IgG.