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Al-Habori, M.; Raman, A.; Lawrence, M. J.; Skett, P.

In VitroEffect of Fenugreek Extracts on Intestinal Sodium-dependent Glucose Uptake and Hepatic Glycogen Phosphorylase A

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  • Media type: E-Article
  • Title: In VitroEffect of Fenugreek Extracts on Intestinal Sodium-dependent Glucose Uptake and Hepatic Glycogen Phosphorylase A
  • Contributor: Al-Habori, M.; Raman, A.; Lawrence, M. J.; Skett, P.
  • imprint: Hindawi Limited, 2001
  • Published in: International Journal of Experimental Diabetes Research
  • Language: English
  • DOI: 10.1155/edr.2001.91
  • ISSN: 1560-4284
  • Origination:
  • Footnote:
  • Description: <jats:p>Fenugreek (<jats:italic>Trigonella foenum-graecum</jats:italic>L. seed) is a food with traditional medicinal use in diabetes. Beneficial effects have been demonstrated in diabetic animals and both insulin-dependent and non-insulin-dependent diabetic subjects. Effects of a lipid extract A, crude ethanolic extract B, further sub-fractions of B (saponin-free C, saponin D and sapogenin E) and a gum fibre fraction F on intestinal sodium-dependent glucose uptake were investigated<jats:italic>in vitro</jats:italic>using rabbit intestinal brush border membrane vesicles. All fractions except A inhibited glucose-uptake at 0.33 and/or 3.3mg/mL (<jats:italic>p</jats:italic>&lt; 0.001). Greatest inhibition was observed with fractions D and E. Diosgenin and trigonelline (compounds reported in fenugreek)also inhibited glucose-uptake (<mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" id="E1"><mml:mrow><mml:msub><mml:mrow><mml:mtext>IC</mml:mtext></mml:mrow><mml:mrow><mml:mn>50</mml:mn></mml:mrow></mml:msub></mml:mrow></mml:math>values approximately 3mg/ml, equivalent to 8mM and 19mM respectively) but did not account for the activity of the crude extracts. Fenugreek extracts had no effect on basal levels of glycogen phosphorylase a (HGPa) activity in rat hepatocyte suspensions. However fractions C and E caused a marginal but statistically significant inhibition (18.9 and 15.1% respectively,<jats:italic>p</jats:italic>&lt; 0.05) of glucagon induction of this enzyme suggesting a glucagon-antagonist effect. Diosgenin (1.65mg/ml; 4mM) inhibited glucagon-induced HGPa activity by 20% (<jats:italic>p</jats:italic>&lt; 0.05), and was more effective than trigonelline (non significant inhibition of 9.4% at 1.65mg/ml, 10mM).</jats:p>
  • Access State: Open Access