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Alizai, Patrick Hamid; Bertram, Lea; Kroy, Daniela; Kummer, Julia; Andert, Anne; Neumann, Ulf Peter; Ulmer, Tom Florian; Fragoulis, Athanassious

Expression of VEGFR-2 during Liver Regeneration after Partial Hepatectomy in a Bioluminescence Mouse Model

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  • Media type: E-Article
  • Title: Expression of VEGFR-2 during Liver Regeneration after Partial Hepatectomy in a Bioluminescence Mouse Model
  • Contributor: Alizai, Patrick Hamid; Bertram, Lea; Kroy, Daniela; Kummer, Julia; Andert, Anne; Neumann, Ulf Peter; Ulmer, Tom Florian; Fragoulis, Athanassious
  • imprint: S. Karger AG, 2017
  • Published in: European Surgical Research
  • Language: English
  • DOI: 10.1159/000479628
  • ISSN: 1421-9921; 0014-312X
  • Origination:
  • Footnote:
  • Description: <jats:p>&lt;b&gt;&lt;i&gt;Background:&lt;/i&gt;&lt;/b&gt; Liver regeneration requires the formation of new blood vessels. Endothelial cell proliferation is stimulated by vascular endothelial growth factor (VEGF) and its receptor tyrosine kinase VEGFR-2. The aim of this study was to investigate VEGFR-2 expression in vivo during liver regeneration after partial hepatectomy (PHx). &lt;b&gt;&lt;i&gt;Methods:&lt;/i&gt;&lt;/b&gt; Transgenic VEGFR-2-luc mice were used in which the luciferase reporter gene was under control of the VEGFR-2 promoter. Following 2/3 PHx, the mice underwent in vivo bioluminescence imaging until the 14th postoperative day. Additionally, liver tissue was analyzed by immunohistochemistry, in vitro luminescence assays, and quantitative RT-PCR. &lt;b&gt;&lt;i&gt;Results:&lt;/i&gt;&lt;/b&gt; In vivo bioluminescence imaging showed a significant increase in VEGFR-2 promoter activity after PHx. Maximum signal was recorded on the 3rd day; 8 days postoperatively the signal intensity decreased significantly. On the 14th day, bioluminescence signal reached almost baseline levels. Immunohistochemistry, quantitative RT-PCR, and in vitro luminescence confirmed a significant increase on the 3rd day following resection. The mRNA expression of VEGFR-2 was significantly higher on day 3 than preoperatively as well as on day 8. &lt;b&gt;&lt;i&gt;Conclusion:&lt;/i&gt;&lt;/b&gt; In vivo bioluminescence imaging with transgenic VEGFR-2-luc mice is feasible and provides a convenient model for noninvasively studying VEGFR-2 expression during liver regeneration. This may facilitate further experiments with modulation of angiogenesis by different substances.</jats:p>