You can manage bookmarks using lists, please log in to your user account for this.
Media type:
E-Article
Title:
Human Mast Cell Sensitization with IgE Increases miRNA-210 Expression
Contributor:
Just, Jesper;
Munk Ipsen, Pernille;
Kruhøffer, Mogens;
Lykkemark, Simon;
Skjold, Tina;
Schiøtz, Peter Oluf;
Hoffmann, Hans Jürgen
Published:
S. Karger AG, 2019
Published in:
International Archives of Allergy and Immunology, 179 (2019) 2, Seite 102-107
Language:
English
DOI:
10.1159/000496513
ISSN:
1018-2438;
1423-0097
Origination:
Footnote:
Description:
Background: MicroRNAs (miRNAs) represent important post-transcriptional regulators with a dynamic expression profile during health and disease. Objectives: We explored the miRNA profile of human mast cells (MCs) during sensitization with IgE, during activation through IgE, and relat ed it to prostaglandin D2 synthesis and histamine release. Method: We investigated the expression pattern of 762 miRNAs during the IgE-mediated sensitization and activation of MCs cultured from CD133+ stem cells that were isolated from allergic asthmatic patients and nonatopic controls. Results: IgE-mediated sensitization increased the expression of miRNA-210 eight-fold. This increase was sustained during IgE-mediated MC activation. Furthermore, we confirmed the increase of the miRNA-132/212 cluster after MC activation. Predicted target genes of miRNA-210/132/212 were enriched in several pathways known to be involved in MC activation. Histamine release was significantly higher in MCs from allergic patients when compared to controls, and a number of miRNAs correlated with histamine release and prostaglandin D2 synthesis during MC activation. Conclusion: The miRNAs and analysis presented here can help to elucidate the role of miRNAs in mediator release during MC activation. We speculate that miRNA-210 could be important in MC sensitization that leads to allergic symptoms.