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Just, Jesper; Munk Ipsen, Pernille; Kruhøffer, Mogens; Lykkemark, Simon; Skjold, Tina; Schiøtz, Peter Oluf; Hoffmann, Hans Jürgen

Human Mast Cell Sensitization with IgE Increases miRNA-210 Expression

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  • Media type: E-Article
  • Title: Human Mast Cell Sensitization with IgE Increases miRNA-210 Expression
  • Contributor: Just, Jesper; Munk Ipsen, Pernille; Kruhøffer, Mogens; Lykkemark, Simon; Skjold, Tina; Schiøtz, Peter Oluf; Hoffmann, Hans Jürgen
  • Published: S. Karger AG, 2019
  • Published in: International Archives of Allergy and Immunology, 179 (2019) 2, Seite 102-107
  • Language: English
  • DOI: 10.1159/000496513
  • ISSN: 1018-2438; 1423-0097
  • Origination:
  • Footnote:
  • Description: Background: MicroRNAs (miRNAs) represent important post-transcriptional regulators with a dynamic expression profile during health and disease. Objectives: We explored the miRNA profile of human mast cells (MCs) during sen­sitization with IgE, during activation through IgE, and relat ed it to prostaglandin D2 synthesis and histamine release. Method: We investigated the expression pattern of 762 miRNAs during the IgE-mediated sensitization and activation of MCs cultured from CD133+ stem cells that were isolated from allergic asthmatic patients and nonatopic controls. Results: IgE-mediated sensitization increased the expression of miRNA-210 eight-fold. This increase was sustained during IgE-mediated MC activation. Furthermore, we confirmed the increase of the miRNA-132/212 cluster after MC activation. Predicted target genes of miRNA-210/132/212 were enriched in several pathways known to be involved in MC activation. Histamine release was significantly higher in MCs from allergic patients when compared to controls, and a number of miRNAs correlated with histamine release and prostaglandin D2 synthesis during MC activation. Conclusion: The miRNAs and analysis presented here can help to elucidate the role of miRNAs in mediator release during MC activation. We speculate that miRNA-210 could be important in MC sensitization that leads to allergic symptoms.