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Hoffmann, Hans Jürgen; Frandsen, Pernille Munk; Christensen, Lars Harder; Schiøtz, Peter Oluf; Dahl, Ronald

Cultured Human Mast Cells Are Heterogeneous for Expression of the High-Affinity IgE Receptor FcεRI

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  • Media type: E-Article
  • Title: Cultured Human Mast Cells Are Heterogeneous for Expression of the High-Affinity IgE Receptor FcεRI
  • Contributor: Hoffmann, Hans Jürgen; Frandsen, Pernille Munk; Christensen, Lars Harder; Schiøtz, Peter Oluf; Dahl, Ronald
  • Published: S. Karger AG, 2012
  • Published in: International Archives of Allergy and Immunology, 157 (2012) 3, Seite 246-250
  • Language: English
  • DOI: 10.1159/000328756
  • ISSN: 1423-0097; 1018-2438
  • Origination:
  • Footnote:
  • Description: <i>Objective:</i> We determined the density of FcΕRI on mast cells cultured from cord (CBMC) and peripheral blood (PBMC) and studied the kinetics of the response through FcΕRI. <i>Methods:</i> Mast cells were cultured from CD133+ progenitors from peripheral or cord blood. FcΕRI was stabilized by culture with 2 µg/ml IgE. Cells were activated by addition of anti-FcΕRI antibody (1 ng/ml–10 µg/ml). Maximal activation, sensitivity, and cooperativity were determined. <i>Results:</i> All cultures were homogeneous for tryptase and metachromasy. All cells expressing FcΕRI could be activated by cross-linking FcΕRI to upregulate CD63. PBMC bind 203,000 molecules of IgE/cell. Stabilization of FcΕRI with IgE doubled the number of CD63+ cells (p = 0.0001) and increased the sensitivity (from 0.083 to 0.013 µg/ml anti-FcΕRI) and the slope factor (from 10.8 to 68) of PBMC but not of CBMC. Anti-IgE reversed these effects (p = 0.0002) but did not reduce activation levels below that of cell lines not stabilized with IgE. <i>Conclusion:</i> Baseline expression of FcΕRI is independent of anti-IgE. The fraction of PBMC that binds high levels of IgE can be activated through FcΕRI.