Description:
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<jats:title>Background</jats:title>
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Urinary 8‐iso‐
<jats:styled-content style="fixed-case">PGF</jats:styled-content>
2α, a marker of oxidative stress, is influenced by the activation of
<jats:styled-content style="fixed-case">NOX</jats:styled-content>
2. It is unclear if platelets 8‐iso‐
<jats:styled-content style="fixed-case">PGF</jats:styled-content>
2α contribute to urinary 8‐iso‐
<jats:styled-content style="fixed-case">PGF</jats:styled-content>
2α.
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<jats:title>Methods and Results</jats:title>
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In a cross‐sectional study, platelet, urinary, and serum 8‐iso‐
<jats:styled-content style="fixed-case">PGF</jats:styled-content>
2α were determined in subjects with downregulation (X‐linked chronic granulomatous disease [
<jats:styled-content style="fixed-case">X‐CGD</jats:styled-content>
], n=25) and upregulation (type
<jats:styled-content style="fixed-case">II</jats:styled-content>
diabetic patients [T2D], n=121) of
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2 and 153 controls matched for sex and age. In diabetic patients (n=18), the above variables were repeated before and after 7 days treatment with 100 mg/day aspirin or 100 mg/day aspirin plus 40 mg/day atorvastatin. In vitro study was performed to see the contribution of blood cells to serum 8‐iso‐
<jats:styled-content style="fixed-case">PGF</jats:styled-content>
2α. Compared with controls,
<jats:styled-content style="fixed-case">X‐CGD</jats:styled-content>
patients had lower platelet, serum, and urinary 8‐iso‐
<jats:styled-content style="fixed-case">PGF</jats:styled-content>
2α values; conversely, diabetic patients had higher values of 8‐iso‐
<jats:styled-content style="fixed-case">PGF</jats:styled-content>
2α compared with controls. Urinary 8‐iso‐
<jats:styled-content style="fixed-case">PGF</jats:styled-content>
2α significantly correlated with both platelet and serum 8‐iso‐
<jats:styled-content style="fixed-case">PGF</jats:styled-content>
2α in the 2 cohorts. A parallel increase of platelet, serum, and urinary 8‐iso‐
<jats:styled-content style="fixed-case">PGF</jats:styled-content>
2α by aspirin and a parallel decrease by aspirin plus atorvastatin were detected in the interventional study. In vitro study demonstrated that platelets contribute to 37% of serum 8‐iso‐
<jats:styled-content style="fixed-case">PGF</jats:styled-content>
2α and that only 13% of it is of extravascular origin.
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<jats:title>Conclusions</jats:title>
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The study suggests that
<jats:styled-content style="fixed-case">NOX</jats:styled-content>
2 contributes to the formation of 8‐iso‐
<jats:styled-content style="fixed-case">PGF</jats:styled-content>
2α in both platelets and urine. The direct correlation between platelet and urinary 8‐iso‐
<jats:styled-content style="fixed-case">PGF</jats:styled-content>
2α suggests that, at least partly, urinary 8‐iso‐
<jats:styled-content style="fixed-case">PGF</jats:styled-content>
2α reflects platelet 8‐iso‐
<jats:styled-content style="fixed-case">PGF</jats:styled-content>
2α production. Analysis of serum 8‐iso‐
<jats:styled-content style="fixed-case">PGF</jats:styled-content>
2α may represent a novel tool to investigate the production of 8‐iso‐
<jats:styled-content style="fixed-case">PGF</jats:styled-content>
2α by blood cells including platelets.
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<jats:title>Clinical Trial Registration</jats:title>
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URL: ClinicalTrials.gov. Unique Identifier:
<jats:ext-link xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="gen" xlink:href="NCT01250340">NCT01250340</jats:ext-link>
.
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