Media type: E-Article Title: IL-2 Adenovector-Transduced Autologous Tumor Cells Induce Antitumor Immune Responses in Patients With Neuroblastoma Contributor: Bowman, Laura; Grossmann, Michael; Rill, Donna; Brown, Michael; Zhong, Wan-yun; Alexander, Barbara; Leimig, Thasia; Coustan-Smith, Elaine; Campana, Dario; Jenkins, Jesse; Woods, Diane; Kitchingman, Geoffrey; Vanin, Elio; Brenner, Malcolm Published: American Society of Hematology, 1998 Published in: Blood, 92 (1998) 6, Seite 1941-1949 Language: English DOI: 10.1182/blood.v92.6.1941.418k03_1941_1949 ISSN: 0006-4971; 1528-0020 Keywords: Cell Biology ; Hematology ; Immunology ; Biochemistry Origination: Footnote: Description: In many different murine models, the immunogenicity of tumor cells can be increased by transduction with a range of immunostimulatory genes, inducing an immune response that causes regression of pre-existing unmodified tumor cells. To investigate the relevance of these animal models to pediatric malignancy, we used autologous unirradiated tumor cells transduced with an adenovirus-IL-2 to immunize 10 children with advanced neuroblastoma. In a dose-escalation study, we found that this tumor immunogen induced a moderate local inflammatory response consisting predominantly of CD4+ T lymphocytes, and a systemic response, with a rise in circulating CD25+and DR+ CD3+ T cells. Patients also made a specific antitumor response, manifest by an IgG antitumor antibody and increased cytotoxic T-cell killing of autologous tumor cells. Clinically, five patients had tumor responses after the tumor immunogen alone (one complete tumor response, one partial response, and three with stable disease). Four of these five patients were shown to have coexisting antitumor cytotoxic activity, as opposed to only one of the patients with nonresponsive disease. These results show a promising correlation between preclinical observations and clinical outcome in this disease, and support further exploration of the approach for malignant diseases of children.© 1998 by The American Society of Hematology. Access State: Open Access