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Bergauer, Annika; Sopel, Nina; Kroß, Bettina; Vuorinen, Tytti; Xepapadaki, Paraskevi; Weiss, Scott T.; Blau, Ashley; Sharma, Himanshu; Kraus, Cornelia; Springel, Rebekka; Rauh, Manfred; Mittler, Susanne; Graser, Anna; Zimmermann, Theodor; Melichar, Volker O.; Kiefer, Alexander; Kowalski, Marek L.; Sobanska, Anna; Jartti, Tuomas; Lukkarinen, Heikki; Papadopoulos, Nikolaos G.; Finotto, Susetta

IFN-α/IFN-λ responses to respiratory viruses in paediatric asthma

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  • Media type: E-Article
  • Title: IFN-α/IFN-λ responses to respiratory viruses in paediatric asthma
  • Contributor: Bergauer, Annika; Sopel, Nina; Kroß, Bettina; Vuorinen, Tytti; Xepapadaki, Paraskevi; Weiss, Scott T.; Blau, Ashley; Sharma, Himanshu; Kraus, Cornelia; Springel, Rebekka; Rauh, Manfred; Mittler, Susanne; Graser, Anna; Zimmermann, Theodor; Melichar, Volker O.; Kiefer, Alexander; Kowalski, Marek L.; Sobanska, Anna; Jartti, Tuomas; Lukkarinen, Heikki; Papadopoulos, Nikolaos G.; Finotto, Susetta
  • imprint: European Respiratory Society (ERS), 2017
  • Published in: European Respiratory Journal
  • Language: English
  • DOI: 10.1183/13993003.00969-2016
  • ISSN: 0903-1936; 1399-3003
  • Keywords: Pulmonary and Respiratory Medicine
  • Origination:
  • Footnote:
  • Description: <jats:p>We analysed the influence of rhinovirus (RV) in nasopharyngeal fluid (NPF) on type I and III interferon (IFN) responses (<jats:italic>e.g.</jats:italic> IFN-α and IFN<jats:bold>-</jats:bold>λ) and their signal transduction, at baseline and during disease exacerbation, in cohorts of pre-school children with and without asthma.</jats:p><jats:p>At the time of recruitment into the Europe-wide study PreDicta, and during symptoms, NPF was collected and the local RV colonisation was analysed. Peripheral blood mononuclear cells (PBMCs) were challenged <jats:italic>in vitro</jats:italic> with RV or not. RNA was analysed by quantitative real-time PCR and gene arrays. Serum was analysed with ELISA for IFNs and C-reactive protein.</jats:p><jats:p>We found that PBMCs from asthmatic children infected <jats:italic>in vitro</jats:italic> with the RV1b serotype upregulated <jats:italic>MYD88</jats:italic>, <jats:italic>IRF1</jats:italic>, <jats:italic>STAT1</jats:italic> and <jats:italic>STAT2</jats:italic> mRNA, whereas <jats:italic>MYD88</jats:italic>, <jats:italic>IRF1</jats:italic>, <jats:italic>STAT1</jats:italic> and <jats:italic>IRF9</jats:italic> were predominantly induced in control children. Moreover, during symptomatic visits because of disease exacerbation associated with RV detection in NPF, IFN-α production was found increased, while IFN-λ secretion was already induced by RV in asthmatic children at baseline.</jats:p><jats:p>During asthma exacerbations associated with RV, asthmatic children can induce IFN-α secretion, indicating a hyperactive immune response to repeated respiratory virus infection.</jats:p>
  • Access State: Open Access