> Details

Baker, Joanne; Ho, Mei-Fong; Pelecanos, Anita; Gatton, Michelle; Chen, Nanhua; Abdullah, Salim; Albertini, Audrey; Ariey, Frederic; Barnwell, John; Bell, David; Cunningham, Jane; Djalle, Djibrine; Echeverry, Diego F; Gamboa, Dionicia; Hii, Jeffery; Kyaw, Myat Phone; Luchavez, Jennifer; Membi, Christopher; Menard, Didier; Murillo, Claribel; Nhem, Sina; Ogutu, Bernhards; Onyor, Pamela; Oyibo, Wellington; [...]

Global sequence variation in the histidine-rich proteins 2 and 3 of Plasmodium falciparum: implications for the performance of malaria rapid diagnostic tests

Reference
management

Bookmarks

Remove from
bookmarks

Share this on Whatsapp

Close

Bookmarks



You can manage bookmarks using lists, please log in to your user account for this.
  • Media type: E-Article
  • Title: Global sequence variation in the histidine-rich proteins 2 and 3 of Plasmodium falciparum: implications for the performance of malaria rapid diagnostic tests
  • Contributor: Baker, Joanne; Ho, Mei-Fong; Pelecanos, Anita; Gatton, Michelle; Chen, Nanhua; Abdullah, Salim; Albertini, Audrey; Ariey, Frederic; Barnwell, John; Bell, David; Cunningham, Jane; Djalle, Djibrine; Echeverry, Diego F; Gamboa, Dionicia; Hii, Jeffery; Kyaw, Myat Phone; Luchavez, Jennifer; Membi, Christopher; Menard, Didier; Murillo, Claribel; Nhem, Sina; Ogutu, Bernhards; Onyor, Pamela; Oyibo, Wellington; [...]
  • imprint: Springer Science and Business Media LLC, 2010
  • Published in: Malaria Journal
  • Language: English
  • DOI: 10.1186/1475-2875-9-129
  • ISSN: 1475-2875
  • Origination:
  • Footnote:
  • Description: <jats:title>Abstract</jats:title> <jats:sec> <jats:title>Background</jats:title> <jats:p>Accurate diagnosis is essential for prompt and appropriate treatment of malaria. While rapid diagnostic tests (RDTs) offer great potential to improve malaria diagnosis, the sensitivity of RDTs has been reported to be highly variable. One possible factor contributing to variable test performance is the diversity of parasite antigens. This is of particular concern for <jats:italic>Plasmodium falciparum</jats:italic> histidine-rich protein 2 (PfHRP2)-detecting RDTs since PfHRP2 has been reported to be highly variable in isolates of the Asia-Pacific region.</jats:p> </jats:sec> <jats:sec> <jats:title>Methods</jats:title> <jats:p>The <jats:italic>pfhrp2</jats:italic> exon 2 fragment from 458 isolates of <jats:italic>P. falciparum</jats:italic> collected from 38 countries was amplified and sequenced. For a subset of 80 isolates, the exon 2 fragment of histidine-rich protein 3 (<jats:italic>pfhrp3</jats:italic>) was also amplified and sequenced. DNA sequence and statistical analysis of the variation observed in these genes was conducted. The potential impact of the <jats:italic>pfhrp2</jats:italic> variation on RDT detection rates was examined by analysing the relationship between sequence characteristics of this gene and the results of the WHO product testing of malaria RDTs: Round 1 (2008), for 34 PfHRP2-detecting RDTs.</jats:p> </jats:sec> <jats:sec> <jats:title>Results</jats:title> <jats:p>Sequence analysis revealed extensive variations in the number and arrangement of various repeats encoded by the genes in parasite populations world-wide. However, no statistically robust correlation between gene structure and RDT detection rate for <jats:italic>P. falciparum</jats:italic> parasites at 200 parasites per microlitre was identified.</jats:p> </jats:sec> <jats:sec> <jats:title>Conclusions</jats:title> <jats:p>The results suggest that despite extreme sequence variation, diversity of PfHRP2 does not appear to be a major cause of RDT sensitivity variation.</jats:p> </jats:sec>
  • Access State: Open Access