Apweiler, Eva;
Sameith, Katrin;
Margaritis, Thanasis;
Brabers, Nathalie;
van de Pasch, Loes;
Bakker, Linda V;
van Leenen, Dik;
Holstege, Frank CP;
Kemmeren, Patrick
Yeast glucose pathways converge on the transcriptional regulation of trehalose biosynthesis
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Media type:
E-Article
Title:
Yeast glucose pathways converge on the transcriptional regulation of trehalose biosynthesis
Contributor:
Apweiler, Eva;
Sameith, Katrin;
Margaritis, Thanasis;
Brabers, Nathalie;
van de Pasch, Loes;
Bakker, Linda V;
van Leenen, Dik;
Holstege, Frank CP;
Kemmeren, Patrick
Published:
Springer Science and Business Media LLC, 2012
Published in:
BMC Genomics, 13 (2012) 1
Language:
English
DOI:
10.1186/1471-2164-13-239
ISSN:
1471-2164
Origination:
Footnote:
Description:
AbstractBackgroundCellular glucose availability is crucial for the functioning of most biological processes. Our understanding of the glucose regulatory system has been greatly advanced by studying the model organismSaccharomyces cerevisiae, but many aspects of this system remain elusive. To understand the organisation of the glucose regulatory system, we analysed 91 deletion mutants of the different glucose signalling and metabolic pathways inSaccharomyces cerevisiaeusing DNA microarrays.ResultsIn general, the mutations do not induce pathway-specific transcriptional responses. Instead, one main transcriptional response is discerned, which varies in direction to mimic either a high or a low glucose response. Detailed analysis uncovers established and new relationships within and between individual pathways and their members. In contrast to signalling components, metabolic components of the glucose regulatory system are transcriptionally more frequently affected. A new network approach is applied that exposes the hierarchical organisation of the glucose regulatory system.ConclusionsThe tight interconnection between the different pathways of the glucose regulatory system is reflected by the main transcriptional response observed. Tps2 and Tsl1, two enzymes involved in the biosynthesis of the storage carbohydrate trehalose, are predicted to be the most downstream transcriptional components. Epistasis analysis oftps2Δ double mutants supports this prediction. Although based on transcriptional changes only, these results suggest that all changes in perceived glucose levels ultimately lead to a shift in trehalose biosynthesis.