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Media type:
E-Article
Title:
Genetic relationships among seven sections of genus Arachisstudied by using SSR markers
Contributor:
Koppolu, Ravi;
Upadhyaya, Hari D;
Dwivedi, Sangam L;
Hoisington, David A;
Varshney, Rajeev K
Published:
Springer Science and Business Media LLC, 2010
Published in:
BMC Plant Biology, 10 (2010) 1
Language:
English
DOI:
10.1186/1471-2229-10-15
ISSN:
1471-2229
Origination:
Footnote:
Description:
AbstractBackgroundThe genusArachis, originated in South America, is divided into nine taxonomical sections comprising of 80 species. Most of theArachisspecies are diploids (2n= 2x= 20) and the tetraploid species (2n= 2x= 40) are found in sectionsArachis,ExtranervosaeandRhizomatosae. Diploid species have great potential to be used as resistance sources for agronomic traits like pests and diseases, drought related traits and different life cycle spans. Understanding of genetic relationships among wild species and between wild and cultivated species will be useful for enhanced utilization of wild species in improving cultivated germplasm. The present study was undertaken to evaluate genetic relationships among species (96 accessions) belonging to seven sections ofArachisby using simple sequence repeat (SSR) markers developed fromArachis hypogaeagenomic library and gene sequences from related genera ofArachis.ResultsThe average transferability rate of 101 SSR markers tested to sectionArachisand six other sections was 81% and 59% respectively. Five markers (IPAHM 164, IPAHM 165, IPAHM 407a, IPAHM 409, and IPAHM 659) showed 100% transferability. Cluster analysis of allelic data from a subset of 32 SSR markers on 85 wild and 11 cultivated accessions grouped accessions according to their genome composition, sections and species to which they belong. A total of 109 species specific alleles were detected in different wild species,Arachis pusillaexhibited largest number of species specific alleles (15). Based on genetic distance analysis, the A-genome accession ICG 8200 (A. duranensis) and the B-genome accession ICG 8206 (A. ipaënsis) were found most closely related toA. hypogaea.ConclusionA set of cross species and cross section transferable SSR markers has been identified that will be useful for genetic studies of wild species ofArachis, including comparative genome mapping, germplasm analysis, population genetic structure and phylogenetic inferences among species. The present study provides strong support based on both genomic and genic markers, probably for the first time, on relationships ofA. monticolaandA. hypogaeaas well as on the most probable donor of A and B-genomes of cultivated groundnut.