> Details

Marcazzan, Sabrina; Braz Carvalho, Marcos J.; Konrad, Matthias; Strangmann, Julia; Tenditnaya, Anna; Baumeister, Theresa; Schmid, Roland M.; Wester, Hans-Jürgen; Ntziachristos, Vasilis; Gorpas, Dimitris; Wang, Timothy C.; Schottelius, Margret; Quante, Michael

CXCR4 peptide-based fluorescence endoscopy in a mouse model of Barrett’s esophagus

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  • Media type: E-Article
  • Title: CXCR4 peptide-based fluorescence endoscopy in a mouse model of Barrett’s esophagus
  • Contributor: Marcazzan, Sabrina; Braz Carvalho, Marcos J.; Konrad, Matthias; Strangmann, Julia; Tenditnaya, Anna; Baumeister, Theresa; Schmid, Roland M.; Wester, Hans-Jürgen; Ntziachristos, Vasilis; Gorpas, Dimitris; Wang, Timothy C.; Schottelius, Margret; Quante, Michael
  • imprint: Springer Science and Business Media LLC, 2022
  • Published in: EJNMMI Research
  • Language: English
  • DOI: 10.1186/s13550-021-00875-7
  • ISSN: 2191-219X
  • Origination:
  • Footnote:
  • Description: <jats:title>Abstract</jats:title><jats:sec> <jats:title>Background</jats:title> <jats:p>Near-infrared (NIR) fluorescence imaging has been emerging as a promising strategy to overcome the high number of early esophageal adenocarcinomas missed by white light endoscopy and random biopsy collection. We performed a preclinical assessment of fluorescence imaging and endoscopy using a novel CXCR4-targeted fluorescent peptide ligand in the L2-IL1B mouse model of Barrett’s esophagus.</jats:p> </jats:sec><jats:sec> <jats:title>Methods</jats:title> <jats:p>Six L2-IL1B mice with advanced stage of disease (12–16 months old) were injected with the CXCR4-targeted, Sulfo-Cy5-labeled peptide (MK007), and ex vivo wide-field imaging of the whole stomach was performed 4 h after injection. Before ex vivo imaging, fluorescence endoscopy was performed in three L2-IL1B mice (12–14 months old)  by a novel imaging system with two L2-IL1B mice used as negative controls.</jats:p> </jats:sec><jats:sec> <jats:title>Results</jats:title> <jats:p>Ex vivo imaging and endoscopy in L2-IL1B mice showed that the CXCR4-targeted MK007 accumulated mostly in the dysplastic lesions with a mean target-to-background ratio &gt; 2. The detection of the Sulfo-Cy5 signal in dysplastic lesions and its co-localization with CXCR4 stained cells  by confocal microscopy further confirmed the imaging results.</jats:p> </jats:sec><jats:sec> <jats:title>Conclusions</jats:title> <jats:p>This preliminary preclinical study shows that CXCR4-targeted fluorescence endoscopy using MK007 can detect dysplastic lesions in a mouse model of Barrett’s esophagus. Further investigations are needed to assess its use in the clinical setting.</jats:p> </jats:sec>
  • Access State: Open Access