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Media type:
E-Article
Title:
Loss of Apc in vivo immediately perturbs Wnt signaling, differentiation, and migration
Contributor:
Sansom, Owen J.;
Reed, Karen R.;
Hayes, Anthony J.;
Ireland, Heather;
Brinkmann, Hannah;
Newton, Ian P.;
Batlle, Eduard;
Simon-Assmann, Patricia;
Clevers, Hans;
Nathke, Inke S.;
Clarke, Alan R.;
Winton, Douglas J.
Published:
Cold Spring Harbor Laboratory, 2004
Published in:
Genes & Development, 18 (2004) 12, Seite 1385-1390
Language:
English
DOI:
10.1101/gad.287404
ISSN:
0890-9369;
1549-5477
Origination:
Footnote:
Description:
Although Apc is well characterized as a tumor-suppressor gene in the intestine, the precise mechanism of this suppression remains to be defined. Using a novel inducible Ahcre transgenic line in conjunction with a loxP-flanked Apc allele we, show that loss of Apc acutely activates Wnt signaling through the nuclear accumulation of β-catenin. Coincidentally, it perturbs differentiation, migration, proliferation, and apoptosis, such that Apc-deficient cells maintain a “crypt progenitor-like” phenotype. Critically, for the first time we confirm a series of Wnt target molecules in an in vivo setting and also identify a series of new candidate targets within the same setting.