Pharmacological characterization of mechanisms involved in the vasorelaxation produced by rosuvastatin in aortic rings from rats with a cafeteria‐style diet
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Media type:
E-Article
Title:
Pharmacological characterization of mechanisms involved in the vasorelaxation produced by rosuvastatin in aortic rings from rats with a cafeteria‐style diet
Description:
<jats:title>Summary</jats:title><jats:p>The present study aimed to investigate the possible influence of several inhibitors and blockers on the vascular effect produced by the acute <jats:italic>in vitro</jats:italic> application of rosuvastatin to phenylephrine‐precontracted aortic rings from rats with a semi‐solid, cafeteria‐style (<jats:styled-content style="fixed-case">CAF</jats:styled-content>) diet. It also aimed to examine the effects of rosuvastatin on the expression of endothelial nitric oxide synthase (<jats:styled-content style="fixed-case">eNOS</jats:styled-content>), inducible nitric oxide synthase, constitutive cyclooxygenase, and inducible cyclooxygenase in aortic rings from rats with a <jats:styled-content style="fixed-case">CAF</jats:styled-content> diet. From comparisons of the effect on phenylephrine‐precontracted aortic rings extracted from rats with two different diets (a standard and a <jats:styled-content style="fixed-case">CAF</jats:styled-content> diet), it was found that 10<jats:sup>−9</jats:sup>–10<jats:sup>−5</jats:sup>‐mol/L rosuvastatin produced lower concentration‐dependent vasorelaxation on rings from the <jats:styled-content style="fixed-case">CAF</jats:styled-content> diet group. The vasorelaxant effect was unaffected by the vehicle, but it was significantly attenuated by 10<jats:sup>−5</jats:sup>‐mol/L <jats:styled-content style="fixed-case"><jats:italic>N</jats:italic><jats:sup>G</jats:sup></jats:styled-content>‐nitro‐<jats:sc>l</jats:sc>‐arginine methyl ester, 10<jats:sup>−2</jats:sup>‐mol/L tetraethylammonium, 10<jats:sup>−3</jats:sup>‐mol/L 4‐aminopyridine, 10<jats:sup>−7</jats:sup>‐mol/L apamin plus 10<jats:sup>−7</jats:sup>‐mol/L charybdotoxin, 10<jats:sup>−5</jats:sup>‐mol/L indomethacin, or 10<jats:sup>−5</jats:sup>‐mol/L cycloheximide. Moreover, in aortic rings from rats with a <jats:styled-content style="fixed-case">CAF</jats:styled-content> diet, rosuvastatin enhanced the expression of <jats:styled-content style="fixed-case">eNOS</jats:styled-content>, inducible nitric oxide synthase, constitutive cyclooxygenase, and inducible cyclooxygenase. The acute <jats:italic>in vitro</jats:italic> application of rosuvastatin to phenylephrine‐precontracted aortic rings from rats with a <jats:styled-content style="fixed-case">CAF</jats:styled-content> diet had a vasorelaxant effect. Overall, the present results suggest that the stimulation of <jats:styled-content style="fixed-case">eNOS</jats:styled-content>, the opening of Ca<jats:sup>2+</jats:sup>‐activated and voltage‐activated K<jats:sup>+</jats:sup> channels, the stimulation of prostaglandin synthesis and enhanced protein levels of <jats:styled-content style="fixed-case">eNOS</jats:styled-content>, inducible nitric oxide synthase, constitutive cyclooxygenase, and inducible cyclooxygenase are involved in this relaxant effect.</jats:p>