> Details

Schäfer, Ingo; Rehbein, Sina; Holtdirk, Annette; Kottmann, Tanja; Klein, Ruth; Müller, Elisabeth; Thoren‐Tolling, Kerstin

Diagnostic cut‐off values for the urinary corticoid:creatinine ratio for the diagnosis of canine Cushing's syndrome using an automated chemiluminescent assay

Reference
management

Bookmarks

Remove from
bookmarks

Share this on Whatsapp

Close

Bookmarks



You can manage bookmarks using lists, please log in to your user account for this.
  • Media type: E-Article
  • Title: Diagnostic cut‐off values for the urinary corticoid:creatinine ratio for the diagnosis of canine Cushing's syndrome using an automated chemiluminescent assay
  • Contributor: Schäfer, Ingo; Rehbein, Sina; Holtdirk, Annette; Kottmann, Tanja; Klein, Ruth; Müller, Elisabeth; Thoren‐Tolling, Kerstin
  • imprint: Wiley, 2023
  • Published in: Veterinary Clinical Pathology
  • Language: English
  • DOI: 10.1111/vcp.13219
  • ISSN: 0275-6382; 1939-165X
  • Keywords: General Veterinary
  • Origination:
  • Footnote:
  • Description: <jats:title>Abstract</jats:title><jats:sec><jats:title>Background</jats:title><jats:p>Cushing's syndrome is one of the most common endocrinopathies in dogs. The preferred screening test for spontaneous Cushing's syndrome is the low‐dose dexamethasone suppression test (LDDST). The diagnostic value of urinary cortisol:creatinine ratio<jats:styled-content>s</jats:styled-content> (UCCR) is questionable.</jats:p></jats:sec><jats:sec><jats:title>Objectives</jats:title><jats:p>The aim of this study was to determine diagnostic cut‐off values for UCCR testing in comparison with LDDST as a clinical reference standard and to calculate the sensitivity and specificity.</jats:p></jats:sec><jats:sec><jats:title>Methods</jats:title><jats:p>Data from 2018 to 2020 were obtained retrospectively from a commercial laboratory. Both LDDST and UCCR were measured by automated chemiluminescent immunoassay (CLIA). The maximum interval between both tests was 14 days. The optimal cut‐off value for UCCR testing was calculated by the Youden index. The sensitivity and specificity of these cut‐off values for the UCCR test and LDDST were assessed by Bayesian latent class models (BLCMs).</jats:p></jats:sec><jats:sec><jats:title>Results</jats:title><jats:p>This study included 324 dogs with both UCCR test and LDDST results. The optimal UCCR cut‐off value, calculated by the Youden index, was 47.4 × 10<jats:sup>−6</jats:sup>. Any UCCR &lt;40 × 10<jats:sup>−6</jats:sup> was interpreted as a negative result, 40–60 × 10<jats:sup>−6</jats:sup> as values in a gray zone, and &gt;60 × 10<jats:sup>−6</jats:sup> as positive. Using the cut‐off of 60 × 10<jats:sup>−6</jats:sup>, BLCM showed 91% (LDDST) and 86% (UCCR test) sensitivity and a specificity of 54% (LDDST) and 63% (UCCR test).</jats:p></jats:sec><jats:sec><jats:title>Conclusions</jats:title><jats:p>Considering an 86% sensitivity and a 63% specificity, UCCR testing may be considered a first‐line investigation to rule out Cushing's syndrome using CLIA analysis. Urine samples can be collected noninvasively at home by the owner, reducing the potential impact of stress.</jats:p></jats:sec>