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Dalsass, Alessia; Mestichelli, Francesca; Ruggieri, Miriana; Gaspari, Paola; Pezzoni, Valerio; Vagnoni, Davide; Angelini, Mario; Angelini, Stefano; Bigazzi, Catia; Falcioni, Sadia; Troiani, Emanuela; Alesiani, Francesco; Catarini, Massimo; Attolico, Immacolata; Scortechini, Ilaria; Discepoli, Giancarlo; Galieni, Piero

6q deletion detected by fluorescence in situ hybridization using bacterial artificial chromosome in chronic lymphocytic leukemia

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  • Media type: E-Article
  • Title: 6q deletion detected by fluorescence in situ hybridization using bacterial artificial chromosome in chronic lymphocytic leukemia
  • Contributor: Dalsass, Alessia; Mestichelli, Francesca; Ruggieri, Miriana; Gaspari, Paola; Pezzoni, Valerio; Vagnoni, Davide; Angelini, Mario; Angelini, Stefano; Bigazzi, Catia; Falcioni, Sadia; Troiani, Emanuela; Alesiani, Francesco; Catarini, Massimo; Attolico, Immacolata; Scortechini, Ilaria; Discepoli, Giancarlo; Galieni, Piero
  • imprint: Wiley, 2013
  • Published in: European Journal of Haematology
  • Language: English
  • DOI: 10.1111/ejh.12115
  • ISSN: 0902-4441; 1600-0609
  • Keywords: Hematology ; General Medicine
  • Origination:
  • Footnote:
  • Description: <jats:title>Abstract</jats:title><jats:p>Deletions of the long arm of chromosome 6 are known to occur at relatively low frequency (3–6%) in chronic lymphocytic leukemia (<jats:styled-content style="fixed-case">CLL</jats:styled-content>), and they are more frequently observed in 6q21. Few data have been reported regarding other bands on 6q involved by cytogenetic alterations in <jats:styled-content style="fixed-case">CLL</jats:styled-content>. The cytogenetic study was performed in nuclei and metaphases obtained after stimulation with a combination of CpG‐oligonucleotide <jats:styled-content style="fixed-case">DSP</jats:styled-content>30 and interleukin‐2. Four bacterial artificial chromosome (<jats:styled-content style="fixed-case">BAC</jats:styled-content>) clones mapping regions in bands 6q16, 6q23, 6q25, 6q27 were used as probes for fluorescence <jats:italic>in situ</jats:italic> hybridization in 107 <jats:styled-content style="fixed-case">CLL</jats:styled-content> cases in order to analyze the occurrence and localization of 6q aberrations. We identified 11 cases (10.2%) with 6q deletion of 107 patients studied with <jats:styled-content style="fixed-case">CLL</jats:styled-content>. The trends of survival curves and the treatment‐free intervals (<jats:styled-content style="fixed-case">TFI</jats:styled-content>) of patients with deletion suggest a better outcome than the other cytogenetic risk groups. We observed two subgroups with 6q deletion as the sole anomaly: two cases with 6q16 deletion, and three cases with 6q25.2–27 deletion. There were differences of age, stage, and <jats:styled-content style="fixed-case">TFI</jats:styled-content> between both subgroups. By using <jats:styled-content style="fixed-case">BAC</jats:styled-content> probes, we observed that 6q deletion has a higher frequency in <jats:styled-content style="fixed-case">CLL</jats:styled-content> and is linked with a good prognosis. In addition, it was observed that the deletion in 6q16 appears to be the most frequent and, if present as the only abnormality, it could be associated with a most widespread disease.</jats:p>