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Media type:
E-Article
Title:
Interaction of spermine with a signal transduction pathway involving phospholipase C, during the growth of Catharanthus roseus transformed roots
Contributor:
Echevarría‐Machado, Ileana;
Ku‐González, Angela;
Loyola‐Vargas, Victor M.;
Hernández‐Sotomayor, S. M. Teresa
Description:
<jats:p> <jats:bold>In <jats:italic>Cantharanthus roseus</jats:italic> transformed roots, the application of methylglyoxal bis(guanylhydrazone) (MGBG), an inhibitor of <jats:italic>S</jats:italic>‐adenosylmethionine decarboxylase (SAMDC; EC 4.1.1.50), inhibited the root growth in a dose‐dependent manner with a DL<jats:sub>50</jats:sub> of about 300 µm. Spermidine and spermine (Spm) levels and SAMDC and phospholipase C (PLC; EC 3.1.4.3) activities were reduced in the presence of the inhibitor. The inhibition was reversed by the addition of Spm. Radioactivity from [<jats:sup>14</jats:sup>C]Spm was detected in an immunoprecipitated fraction with an antibody anti‐PLC‐δ. To our knowledge, this is the first direct evidence that demonstrates an interaction of Spm with the signal transduction cascade phosphoinositide‐Ca<jats:sup>2+</jats:sup>.</jats:bold> </jats:p>