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Media type:
E-Article
Title:
Nitrogen Fixation by Extracts of Mycobacterium flavum 301 : Use of Natural Electron Donors and Oxygen‐Sensitivity of Cell‐Free Preparations
:
Use of Natural Electron Donors and Oxygen‐Sensitivity of Cell‐Free Preparations
Description:
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<jats:list-item><jats:p>Crude extracts of lactate‐grown <jats:italic>Mycobacterium flavum</jats:italic> 301 reduced acetylene to ethylene with NADH as electron donor provided benzyl viologen was present; ATP was required. Lactate, pyruvate, H<jats:sub>2</jats:sub> or glucose‐6‐phosphate were also active donors <jats:italic>via</jats:italic> appropriate dehydrogenases.</jats:p></jats:list-item>
<jats:list-item><jats:p>Crude extracts also reduced cyanide to methane with NADH, ATP and benzyl viologen.</jats:p></jats:list-item>
<jats:list-item><jats:p>Debris (composed of wall and membrane fragments) had lactic, pyruvic and NADH dehydrogenase activities. They reduced acetylene with sodium dithionite or NADH. Benzyl viologen was not required with NADH; ATP and O<jats:sub>2</jats:sub> inhibited the reaction.</jats:p></jats:list-item>
<jats:list-item><jats:p>Crude extracts were as sensitive to inactivation by oxygen as extracts from anaerobic bacteria. Glutathione decreased and ATP increased oxygen‐sensitivity.</jats:p></jats:list-item>
<jats:list-item><jats:p>Debris preparations were not inactivated by oxygen.</jats:p></jats:list-item>
<jats:list-item><jats:p>These results suggest that the nitrogen‐fixing system of <jats:italic>M. flavum</jats:italic> 301 resembles those of other aerobes, but that its mechanisms for protecting nitrogenase from oxygen are more primitive.</jats:p></jats:list-item>
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