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Media type:
E-Article
Title:
Pro‐atherogenic lung and oral pathogens induce an inflammatory response in human and mouse mast cells
Contributor:
Oksaharju, Anna;
Lappalainen, Jani;
Tuomainen, Anita M.;
Pussinen, Pirkko J.;
Puolakkainen, Mirja;
Kovanen, Petri T.;
Lindstedt, Ken A.
Published:
Wiley, 2009
Published in:
Journal of Cellular and Molecular Medicine, 13 (2009) 1, Seite 103-113
Language:
English
DOI:
10.1111/j.1582-4934.2008.00285.x
ISSN:
1582-1838;
1582-4934
Origination:
Footnote:
Description:
AbstractA broad variety of microbes are present in atherosclerotic plaques and chronic bacterial infection increases the risk of atherosclerosis by mechanisms that have remained vague. One possible mechanism is that bacteria or bacterial products activate plaque mast cells that are known to participate in the pathogenesis of atherosclerosis. Here, we show by real‐time PCR analysis and ELISA that Chlamydia pneumoniae (Cpn) and a periodontal pathogen, Aggregatibacter actinomycetemcomitans (Aa), both induce a time and concentration‐dependent expression and secretion of interleukin 8 (IL‐8), tumour necrosis factor‐α (TNF‐α) and monocyte chemoattractant protein‐1 (MCP‐1) by cultured human peripheral blood‐derived mast cells, but not anti‐inflammatory molecules, such as IL‐10 or transforming growth factor β1 (TGF‐β1). The IL‐8 and MCP‐1 responses were immediate, whereas the onset of TNF‐α secretion was delayed. The Cpn‐mediated pro‐inflammatory effect was attenuated when the bacteria were inactivated by UV‐treatment. Human monocyte‐derived macrophages that were pre‐infected with Cpn also induced a significant pro‐inflammatory response in human mast cells, both in cocultures and when preconditioned media from Cpn‐infected macrophages were used. Intranasal and intravenous administration of live Cpn and Aa, respectively induced an accumulation of activated mast cells in the aortic sinus of apolipoprotein E‐deficient mice, however, with varying responses in the systemic levels of lipopolysaccharide (LPS) and TNF‐α. Pro‐atherogenic Cpn and Aa induce a pro‐inflammatory response in cultured human connective tissue‐type mast cells and activation of mouse aortic mast cells in vivo.