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Media type:
E-Article
Title:
Effect of Streptococcus parvulus and Peptostreptococcus magnus on Cytotoxin Levels of Clostridium difficile in Anaerobic Continuous Flow Culture
Description:
<jats:title>Abstract</jats:title><jats:p>An anaerobic continuous flow (CF) culture method was used in order to study the effect of <jats:italic>Peptostreptococcus magnus</jats:italic> and <jats:italic>Streptococcus parvulus</jats:italic>, anaerobic gram‐positive cocci which are members of intestinal bacterial flora, on growth and cytotoxin‐activity of <jats:italic>Clostridium difficile.</jats:italic> The growth‐ and the cytotoxin activity‐patterns of <jats:italic>C. difficile</jats:italic> in an established CF culture of <jats:italic>P. magnus</jats:italic> were similar to those of <jats:italic>C. difficile</jats:italic> alone. On the other hand, in the mixed culture system of <jats:italic>C. difficile</jats:italic> and <jats:italic>S. parvulus</jats:italic>, the cytotoxin levels were significantly lower as compared with <jats:italic>C. difficile</jats:italic> alone in spite of the fact that no differences existed between growth of <jats:italic>C. difficile</jats:italic> in mixed and single culture systems. The culture filtrate of <jats:italic>P. magnus</jats:italic> did not influence the growth and cytotoxin production of <jats:italic>C. difficile</jats:italic>, nor did that of <jats:italic>S. parvulus</jats:italic> have any effect on growth of <jats:italic>C. difficile</jats:italic> in static culture. The cytotoxin activity of <jats:italic>C. difficile</jats:italic> was, however, suppressed by the culture filtrate of <jats:italic>S. parvulus.</jats:italic> Furthermore, when <jats:italic>P. magnus</jats:italic> or <jats:italic>S. parvulus</jats:italic> was statically cultured in a medium containing cytotoxic culture filtrate of <jats:italic>C. difficile</jats:italic>, the toxin in the medium was not inactivated.</jats:p>