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Media type:
E-Article
Title:
Spatial dissection of the cis‐ and trans‐Golgi compartments in the malaria parasite Plasmodium falciparum
Contributor:
Struck, Nicole S.;
Herrmann, Susann;
Schmuck‐Barkmann, Iris;
De Souza Dias, Suzana;
Haase, Silvia;
Cabrera, Ana L.;
Treeck, Moritz;
Bruns, Caroline;
Langer, Christine;
Cowman, Alan F.;
Marti, Matthias;
Spielmann, Tobias;
Gilberger, Tim W.
imprint:
Wiley, 2008
Published in:Molecular Microbiology
Language:
English
DOI:
10.1111/j.1365-2958.2008.06125.x
ISSN:
0950-382X;
1365-2958
Origination:
Footnote:
Description:
<jats:title>Summary</jats:title><jats:p>The Golgi apparatus forms the heart of the secretory pathway in eukaryotic cells where proteins are modified, processed and sorted. The transport of proteins from the endoplasmic reticulum (ER) to the <jats:italic>cis‐</jats:italic>side of the Golgi complex takes place at specialized ER sub‐domains known as transitional ER (tER). We used the <jats:italic>Plasmodium falciparum</jats:italic> orthologue of Sec13p to analyse tER organization. We show that the distribution of <jats:italic>Pf</jats:italic>Sec13p is restricted to defined areas of the ER membrane. These foci are juxtaposed to the Golgi apparatus and might represent tER sites. To further analyse <jats:italic>cis</jats:italic>‐ to <jats:italic>trans</jats:italic>‐Golgi architecture, we generated a double transfectant parasite line that expresses the Golgi marker Golgi reassembly stacking protein (GRASP) as a green fluorescent protein fusion and the <jats:italic>trans‐</jats:italic>Golgi marker Rab6 as a DsRed fusion protein. Our data demonstrate that Golgi multiplication is closely linked to tER multiplication, and that parasite maturation is accompanied by the spatial separation of the <jats:italic>cis‐</jats:italic> and <jats:italic>trans‐</jats:italic> face of this organelle.</jats:p>