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Media type:
E-Article
Title:
AToxoplasma gondiimutant highlights the importance of translational regulation in the apicoplast during animal infection
Contributor:
Payne, T. Matthew;
Payne, Amanda J.;
Knoll, Laura J.
Published:
Wiley, 2011
Published in:
Molecular Microbiology, 82 (2011) 5, Seite 1204-1216
Language:
English
DOI:
10.1111/j.1365-2958.2011.07879.x
ISSN:
0950-382X;
1365-2958
Origination:
Footnote:
Description:
SummaryToxoplasma gondiiis an obligate intracellular parasite of all warm‐blooded animals. We previously described a forward genetic screen to identifyT. gondiimutants defective in the establishment of a chronic infection. One of the mutants isolated was disrupted in the 3′ untranslated region (3′UTR) of an orthologue of bacterial translation elongation factor G (EFG). The mutant does not have a growth defect in tissue culture. Genetic complementation of this mutant with the genomic locus ofTgEFGrestores virulence in an acute infection mouse model. Epitope tagged TgEFG localized to the apicoplast, via a non‐canonical targeting signal, where it functions as an elongation factor for translation in the apicoplast. Comparisons of TgEFG expression constructs with wild‐type or mutant 3′UTRs showed that a wild‐type 3′UTR is necessary for translation of TgEFG. In tissue culture, theTgEFGtranscript is equally abundant in wild‐type and mutant parasites; however, during an animal infection, theTgEFGtranscript is increased more than threefold in the mutant. These results highlight that in tissue culture, translation in the apicoplast can be diminished, but during an animal infection, translation in the apicoplast must be fully functional.