Schweiger, Wolfgang;
Steiner, Barbara;
Ametz, Christian;
Siegwart, Gerald;
Wiesenberger, Gerlinde;
Berthiller, Franz;
Lemmens, Marc;
Jia, Haiyan;
Adam, Gerhard;
Muehlbauer, Gary J.;
Kreil, David P.;
Buerstmayr, Hermann
Transcriptomic characterization of two major Fusarium resistance quantitative trait loci (QTLs), Fhb1 and Qfhs.ifa‐5A, identifies novel candidate genes
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Media type:
E-Article
Title:
Transcriptomic characterization of two major Fusarium resistance quantitative trait loci (QTLs), Fhb1 and Qfhs.ifa‐5A, identifies novel candidate genes
Contributor:
Schweiger, Wolfgang;
Steiner, Barbara;
Ametz, Christian;
Siegwart, Gerald;
Wiesenberger, Gerlinde;
Berthiller, Franz;
Lemmens, Marc;
Jia, Haiyan;
Adam, Gerhard;
Muehlbauer, Gary J.;
Kreil, David P.;
Buerstmayr, Hermann
imprint:
Wiley, 2013
Published in:Molecular Plant Pathology
Language:
English
DOI:
10.1111/mpp.12048
ISSN:
1464-6722;
1364-3703
Origination:
Footnote:
Description:
<jats:title>Summary</jats:title><jats:p>Fusarium head blight, caused by <jats:italic><jats:styled-content style="fixed-case">F</jats:styled-content>usarium graminearum</jats:italic>, is a devastating disease of wheat. We developed near‐isogenic lines (<jats:styled-content style="fixed-case">NILs</jats:styled-content>) differing in the two strongest known <jats:italic><jats:styled-content style="fixed-case">F</jats:styled-content>. graminearum</jats:italic> resistance quantitative trait loci (<jats:styled-content style="fixed-case">QTL</jats:styled-content>s), <jats:italic><jats:styled-content style="fixed-case">Q</jats:styled-content>fhs.ndsu‐<jats:styled-content style="fixed-case">3BS</jats:styled-content></jats:italic> (also known as resistance gene <jats:italic><jats:styled-content style="fixed-case">Fhb1</jats:styled-content></jats:italic>) and <jats:italic><jats:styled-content style="fixed-case">Q</jats:styled-content>fhs.ifa‐<jats:styled-content style="fixed-case">5A</jats:styled-content></jats:italic>, which are located on the short arm of chromosome <jats:styled-content style="fixed-case">3B</jats:styled-content> and on chromosome <jats:styled-content style="fixed-case">5A</jats:styled-content>, respectively. These <jats:styled-content style="fixed-case">NILs</jats:styled-content> showing different levels of resistance were used to identify transcripts that are changed significantly in a <jats:styled-content style="fixed-case">QTL</jats:styled-content>‐specific manner in response to the pathogen and between mock‐inoculated samples. After inoculation with <jats:italic><jats:styled-content style="fixed-case">F</jats:styled-content>. graminearum</jats:italic> spores, 16 transcripts showed a significantly different response for <jats:italic><jats:styled-content style="fixed-case">Fhb1</jats:styled-content></jats:italic> and 352 for <jats:italic><jats:styled-content style="fixed-case">Qfhs</jats:styled-content>.ifa‐<jats:styled-content style="fixed-case">5A</jats:styled-content></jats:italic>. Notably, we identified a lipid transfer protein which is constitutively at least 50‐fold more abundant in plants carrying the resistant allele of <jats:italic><jats:styled-content style="fixed-case">Qfhs</jats:styled-content>.ifa‐<jats:styled-content style="fixed-case">5A</jats:styled-content></jats:italic>. In addition to this candidate gene associated with <jats:italic><jats:styled-content style="fixed-case">Qfhs</jats:styled-content>.ifa‐<jats:styled-content style="fixed-case">5A</jats:styled-content></jats:italic>, we identified a uridine diphosphate (<jats:styled-content style="fixed-case">UDP</jats:styled-content>)‐glycosyltransferase gene, designated <jats:italic><jats:styled-content style="fixed-case">TaUGT12887</jats:styled-content></jats:italic>, exhibiting a positive difference in response to the pathogen in lines harbouring both <jats:styled-content style="fixed-case">QTL</jats:styled-content>s relative to lines carrying only the <jats:italic><jats:styled-content style="fixed-case">Qfhs</jats:styled-content>.ifa‐<jats:styled-content style="fixed-case">5A</jats:styled-content></jats:italic> resistance allele, suggesting <jats:italic><jats:styled-content style="fixed-case">Fhb1</jats:styled-content></jats:italic> dependence of this transcript. Yet, this dependence was observed only in the <jats:styled-content style="fixed-case">NIL</jats:styled-content> with already higher basal resistance. The complete <jats:styled-content style="fixed-case">cDNA</jats:styled-content> of <jats:italic><jats:styled-content style="fixed-case">TaUGT12887</jats:styled-content></jats:italic> was reconstituted from available wheat genomic sequences, and a synthetic recoded gene was expressed in a toxin‐sensitive strain of <jats:italic><jats:styled-content style="fixed-case">S</jats:styled-content>accharomyces cerevisiae</jats:italic>. This gene conferred deoxynivalenol resistance, albeit much weaker than that observed with the previously characterized barley <jats:styled-content style="fixed-case">HvUGT13248</jats:styled-content>.</jats:p>