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Patron, Nicola J.; Orzaez, Diego; Marillonnet, Sylvestre; Warzecha, Heribert; Matthewman, Colette; Youles, Mark; Raitskin, Oleg; Leveau, Aymeric; Farré, Gemma; Rogers, Christian; Smith, Alison; Hibberd, Julian; Webb, Alex A. R.; Locke, James; Schornack, Sebastian; Ajioka, Jim; Baulcombe, David C.; Zipfel, Cyril; Kamoun, Sophien; Jones, Jonathan D. G.; Kuhn, Hannah; Robatzek, Silke; Van Esse, H. Peter; Sanders, Dale; [...]

Standards for plant synthetic biology: a common syntax for exchange of DNA parts

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  • Media type: E-Article
  • Title: Standards for plant synthetic biology: a common syntax for exchange of DNA parts
  • Contributor: Patron, Nicola J.; Orzaez, Diego; Marillonnet, Sylvestre; Warzecha, Heribert; Matthewman, Colette; Youles, Mark; Raitskin, Oleg; Leveau, Aymeric; Farré, Gemma; Rogers, Christian; Smith, Alison; Hibberd, Julian; Webb, Alex A. R.; Locke, James; Schornack, Sebastian; Ajioka, Jim; Baulcombe, David C.; Zipfel, Cyril; Kamoun, Sophien; Jones, Jonathan D. G.; Kuhn, Hannah; Robatzek, Silke; Van Esse, H. Peter; Sanders, Dale; [...]
  • Published: Wiley, 2015
  • Published in: New Phytologist, 208 (2015) 1, Seite 13-19
  • Language: English
  • DOI: 10.1111/nph.13532
  • ISSN: 0028-646X; 1469-8137
  • Origination:
  • Footnote:
  • Description: <jats:title>Summary</jats:title><jats:p>Inventors in the field of mechanical and electronic engineering can access multitudes of components and, thanks to standardization, parts from different manufacturers can be used in combination with each other. The introduction of BioBrick standards for the assembly of characterized <jats:styled-content style="fixed-case">DNA</jats:styled-content> sequences was a landmark in microbial engineering, shaping the field of synthetic biology. Here, we describe a standard for Type <jats:styled-content style="fixed-case">IIS</jats:styled-content> restriction endonuclease‐mediated assembly, defining a common syntax of 12 fusion sites to enable the facile assembly of eukaryotic transcriptional units. This standard has been developed and agreed by representatives and leaders of the international plant science and synthetic biology communities, including inventors, developers and adopters of Type <jats:styled-content style="fixed-case">IIS</jats:styled-content> cloning methods. Our vision is of an extensive catalogue of standardized, characterized <jats:styled-content style="fixed-case">DNA</jats:styled-content> parts that will accelerate plant bioengineering.</jats:p>
  • Access State: Open Access