Verhoeven, Koen J. F.;
Verbon, Eline H.;
van Gurp, Thomas P.;
Oplaat, Carla;
Ferreira de Carvalho, Julie;
Morse, Alison M.;
Stahl, Mark;
Macel, Mirka;
McIntyre, Lauren M.
Intergenerational environmental effects: functional signals in offspring transcriptomes and metabolomes after parental jasmonic acid treatment in apomictic dandelion
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Media type:
E-Article
Title:
Intergenerational environmental effects: functional signals in offspring transcriptomes and metabolomes after parental jasmonic acid treatment in apomictic dandelion
Contributor:
Verhoeven, Koen J. F.;
Verbon, Eline H.;
van Gurp, Thomas P.;
Oplaat, Carla;
Ferreira de Carvalho, Julie;
Morse, Alison M.;
Stahl, Mark;
Macel, Mirka;
McIntyre, Lauren M.
Description:
<jats:title>Summary</jats:title><jats:p>
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<jats:list-item><jats:p>Parental environments can influence offspring traits. However, the magnitude of the impact of parental environments on offspring molecular phenotypes is poorly understood. Here, we test the direct effects and intergenerational effects of jasmonic acid (<jats:styled-content style="fixed-case">JA</jats:styled-content>) treatment, which is involved in herbivory‐induced defense signaling, on transcriptomes and metabolomes in apomictic common dandelion (<jats:italic>Taraxacum officinale</jats:italic>).</jats:p></jats:list-item>
<jats:list-item><jats:p>In a full factorial crossed design with parental and offspring <jats:styled-content style="fixed-case">JA</jats:styled-content> and control treatments, we performed leaf <jats:styled-content style="fixed-case">RNA</jats:styled-content>‐seq gene expression analysis, <jats:styled-content style="fixed-case">LC</jats:styled-content>‐<jats:styled-content style="fixed-case">MS</jats:styled-content> metabolomics and total phenolics assays in offspring plants.</jats:p></jats:list-item>
<jats:list-item><jats:p>Expression analysis, leveraged by a <jats:italic>de novo</jats:italic> assembled transcriptome, revealed an induced response to <jats:styled-content style="fixed-case">JA</jats:styled-content> exposure that is consistent with known <jats:styled-content style="fixed-case">JA</jats:styled-content> effects. The intergenerational effect of treatment was considerable: 307 of 858 detected <jats:styled-content style="fixed-case">JA</jats:styled-content>‐responsive transcripts were affected by parental <jats:styled-content style="fixed-case">JA</jats:styled-content> treatment. In terms of the numbers of metabolites affected, the magnitude of the chemical response to parental <jats:styled-content style="fixed-case">JA</jats:styled-content> exposure was <jats:italic>c</jats:italic>. 10% of the direct <jats:styled-content style="fixed-case">JA</jats:styled-content> treatment response. Transcriptome and metabolome analyses both identified the phosphatidylinositol signaling pathway as a target of intergenerational <jats:styled-content style="fixed-case">JA</jats:styled-content> effects.</jats:p></jats:list-item>
<jats:list-item><jats:p>Our results highlight that parental environments can have substantial effects in offspring generations. Transcriptome and metabolome assays provide a basis for zooming in on the potential mechanisms of inherited <jats:styled-content style="fixed-case">JA</jats:styled-content> effects.</jats:p></jats:list-item>
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