> Details

Müller, Yannic; Patwari, Payal; Stöcker, Tyll; Zeisler‐Diehl, Viktoria; Steiner, Ulrike; Campoli, Chiara; Grewe, Lea; Kuczkowska, Magdalena; Dierig, Maya Marita; Jose, Sarah; Hetherington, Alistair M.; Acosta, Ivan F.; Schoof, Heiko; Schreiber, Lukas; Dörmann, Peter

Isolation and characterization of the gene HvFAR1 encoding acyl‐CoA reductase from the cer‐za.227 mutant of barley (Hordeum vulgare) and analysis of the cuticular barrier functions

Reference
management

Bookmarks

Remove from
bookmarks

Share this on Whatsapp

Close

Bookmarks



You can manage bookmarks using lists, please log in to your user account for this.
  • Media type: E-Article
  • Title: Isolation and characterization of the gene HvFAR1 encoding acyl‐CoA reductase from the cer‐za.227 mutant of barley (Hordeum vulgare) and analysis of the cuticular barrier functions
  • Contributor: Müller, Yannic; Patwari, Payal; Stöcker, Tyll; Zeisler‐Diehl, Viktoria; Steiner, Ulrike; Campoli, Chiara; Grewe, Lea; Kuczkowska, Magdalena; Dierig, Maya Marita; Jose, Sarah; Hetherington, Alistair M.; Acosta, Ivan F.; Schoof, Heiko; Schreiber, Lukas; Dörmann, Peter
  • imprint: Wiley, 2023
  • Published in: New Phytologist, 239 (2023) 5, Seite 1903-1918
  • Language: English
  • DOI: 10.1111/nph.19063
  • ISSN: 0028-646X; 1469-8137
  • Origination:
  • Footnote:
  • Description: <jats:title>Summary</jats:title><jats:p> <jats:list list-type="bullet"> <jats:list-item><jats:p>The cuticle is a protective layer covering aerial plant organs. We studied the function of waxes for the establishment of the cuticular barrier in barley (<jats:italic>Hordeum vulgare</jats:italic>). The barley <jats:italic>eceriferum</jats:italic> mutants <jats:italic>cer‐za.227</jats:italic> and <jats:italic>cer‐ye.267</jats:italic> display reduced wax loads, but the genes affected, and the consequences of the wax changes for the barrier function remained unknown.</jats:p></jats:list-item> <jats:list-item><jats:p>Cuticular waxes and permeabilities were measured in <jats:italic>cer‐za.227</jats:italic> and <jats:italic>cer‐ye.267</jats:italic>. The mutant loci were isolated by bulked segregant RNA sequencing. New <jats:italic>cer‐za</jats:italic> alleles were generated by genome editing. The CER‐ZA protein was characterized after expression in yeast and Arabidopsis <jats:italic>cer4‐3</jats:italic>.</jats:p></jats:list-item> <jats:list-item><jats:p><jats:italic>Cer‐za.227</jats:italic> carries a mutation in <jats:italic>HORVU5Hr1G089230</jats:italic> encoding acyl‐CoA reductase (FAR1). The <jats:italic>cer‐ye.267</jats:italic> mutation is located to <jats:italic>HORVU4Hr1G063420</jats:italic> encoding β‐ketoacyl‐CoA synthase (KAS1) and is allelic to <jats:italic>cer‐zh.54</jats:italic>. The amounts of intracuticular waxes were strongly decreased in <jats:italic>cer‐ye.267</jats:italic>. The cuticular water loss and permeability of <jats:italic>cer‐za.227</jats:italic> were similar to wild‐type (WT), but were increased in <jats:italic>cer‐ye.267.</jats:italic> Removal of epicuticular waxes revealed that intracuticular, but not epicuticular waxes are required to regulate cuticular transpiration.</jats:p></jats:list-item> <jats:list-item><jats:p>The differential decrease in intracuticular waxes between <jats:italic>cer‐za.227</jats:italic> and <jats:italic>cer‐ye.267</jats:italic>, and the removal of epicuticular waxes indicate that the cuticular barrier function mostly depends on the presence of intracuticular waxes.</jats:p></jats:list-item> </jats:list> </jats:p>