Description:
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Several genetically encoded sensors have been developed to study live cell NADPH/NADP
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dynamics, but their use has been predominantly in vitro. Here, we developed an in vivo assay using the Apollo-NADP
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sensor and microfluidic devices to measure endogenous NADPH/NADP
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dynamics in the pancreatic β cells of live zebrafish embryos. Flux through the pentose phosphate pathway, the main source of NADPH in many cell types, has been reported to be low in β cells. Thus, it is unclear how these cells compensate to meet NADPH demands. Using our assay, we show that pyruvate cycling is the main source of NADP
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reduction in β cells, with contributions from folate cycling after acute electrical activation. INS1E β cells also showed a stress-induced increase in folate cycling and further suggested that this cycling requires both increased glycolytic intermediates and cytosolic NAD
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. Overall, we show in vivo application of the Apollo-NADP
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sensor and reveal that β cells are capable of adapting NADPH/NADP
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redox during stress.
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