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Media type:
E-Article
Title:
Simultaneous analysis of pMHC binding and reactivity unveils virus-specific CD8 T cell immunity to a concise epitope set
Contributor:
Kristensen, Nikolaj Pagh;
Dionisio, Edoardo;
Bentzen, Amalie Kai;
Tamhane, Tripti;
Kemming, Janine Sophie;
Nos, Grigorii;
Voss, Lasse Frank;
Hansen, Ulla Kring;
Lauer, Georg Michael;
Hadrup, Sine Reker
Published:
American Association for the Advancement of Science (AAAS), 2024
Published in:
Science Advances, 10 (2024) 15
Language:
English
DOI:
10.1126/sciadv.adm8951
ISSN:
2375-2548
Origination:
Footnote:
Description:
<jats:p>CD8 T cells provide immunity to virus infection through recognition of epitopes presented by peptide major histocompatibility complexes (pMHCs). To establish a concise panel of widely recognized T cell epitopes from common viruses, we combined analysis of TCR down-regulation upon stimulation with epitope-specific enumeration based on barcode-labeled pMHC multimers. We assess CD8 T cell binding and reactivity for 929 previously reported epitopes in the context of 1 of 25 HLA alleles representing 29 viruses. The prevalence and magnitude of CD8 T cell responses were evaluated in 48 donors and reported along with 137 frequently recognized virus epitopes, many of which were underrepresented in the public domain. Eighty-four percent of epitope-specific CD8 T cell populations demonstrated reactivity to peptide stimulation, which was associated with effector and long-term memory phenotypes. Conversely, nonreactive T cell populations were associated primarily with naive phenotypes. Our analysis provides a reference map of epitopes for characterizing CD8 T cell responses toward common human virus infections.</jats:p>