Stokoe, David;
Stephens, Leonard R.;
Copeland, Terry;
Gaffney, Piers R. J.;
Reese, Colin B.;
Painter, Gavin F.;
Holmes, Andrew B.;
McCormick, Frank;
Hawkins, Phillip T.
Dual Role of Phosphatidylinositol-3,4,5-trisphosphate in the Activation of Protein Kinase B
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Media type:
E-Article
Title:
Dual Role of Phosphatidylinositol-3,4,5-trisphosphate in the Activation of Protein Kinase B
Contributor:
Stokoe, David;
Stephens, Leonard R.;
Copeland, Terry;
Gaffney, Piers R. J.;
Reese, Colin B.;
Painter, Gavin F.;
Holmes, Andrew B.;
McCormick, Frank;
Hawkins, Phillip T.
Published:
American Association for the Advancement of Science (AAAS), 1997
Published in:
Science, 277 (1997) 5325, Seite 567-570
Language:
English
DOI:
10.1126/science.277.5325.567
ISSN:
0036-8075;
1095-9203
Origination:
Footnote:
Description:
<jats:p>
Protein kinase B (PKB) is a proto-oncogene that is activated in signaling pathways initiated by phosphoinositide 3-kinase. Chromatographic separation of brain cytosol revealed a kinase activity that phosphorylated and activated PKB only in the presence of phosphatidylinositol-3,4,5-trisphosphate [PtdIns(3,4,5)P
<jats:sub>3</jats:sub>
]. Phosphorylation occurred exclusively on threonine-308, a residue implicated in activation of PKB in vivo. PtdIns(3,4,5)P
<jats:sub>3</jats:sub>
was determined to have a dual role: Its binding to the pleckstrin homology domain of PKB was required to allow phosphorylation by the upstream kinase and it directly activated the upstream kinase.
</jats:p>