You can manage bookmarks using lists, please log in to your user account for this.
Media type:
E-Article
Title:
Drosophila Mitotic Domain Boundaries as Cell Fate Boundaries
Contributor:
Cambridge, Sidney B.;
Davis, Robert L.;
Minden, Jonathan S.
Published:
American Association for the Advancement of Science (AAAS), 1997
Published in:
Science, 277 (1997) 5327, Seite 825-828
Language:
English
DOI:
10.1126/science.277.5327.825
ISSN:
0036-8075;
1095-9203
Origination:
Footnote:
Description:
Fate determination in Drosophila embryos is evidenced by the appearance of mitotic domains. To identify fate or fates of cells, individual cells in mitotic domains 2, 8, and 15 were marked and monitored through development. Comparison of the different fates indicated that domain boundaries are cell fate boundaries. Cells were marked by expression of GAL4-dependent transgenes after photoactivation of a caged GAL4VP16 analog that had its DNA binding activity inhibited with a photolabile blocking reagent. Caged GAL4VP16 was also used to induce gene expression in Xenopus embryos. Thus, photoactivated gene expression is a versatile tool for spatiotemporal control of gene expression.