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Media type:
E-Article
Title:
The "Spliceosome": Yeast Pre-Messenger RNA Associates with a 40 S Complex in a Splicing-Dependent Reaction
Contributor:
Brody, Edward;
Abelson, John
Published:
American Association for the Advancement of Science (AAAS), 1985
Published in:
Science, 228 (1985) 4702, Seite 963-967
Language:
English
DOI:
10.1126/science.3890181
ISSN:
0036-8075;
1095-9203
Origination:
Footnote:
Description:
The in vitro splicing reactions of pre-messenger RNA (pre-mRNA) in a yeast extract were analyzed by glycerol gradient centrifugation. Labeled pre-mRNA appears in a 40 S peak only if the pre-mRNA undergoes the first of the two partial splicing reactions. RNA analysis after extraction of glycerol gradient fractions shows that lariat-form intermediates, molecules that occur only in mRNA splicing, are found almost exclusively in this 40 S complex. Another reaction intermediate, cut 5′ exon RNA, can also be found concentrated in this complex. The complex is stable even in 400 m M KCl, although at this salt concentration, it sediments at 35 S and is clearly distinguishable from 40 S ribosomal subunits. This complex, termed a "spliceosome," is thought to contain components necessary for mRNA splicing; its existence can explain how separated exons on pre-mRNA are brought into contact.