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Brody, Edward; Abelson, John

The "Spliceosome": Yeast Pre-Messenger RNA Associates with a 40 S Complex in a Splicing-Dependent Reaction

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  • Media type: E-Article
  • Title: The "Spliceosome": Yeast Pre-Messenger RNA Associates with a 40 S Complex in a Splicing-Dependent Reaction
  • Contributor: Brody, Edward; Abelson, John
  • Published: American Association for the Advancement of Science (AAAS), 1985
  • Published in: Science, 228 (1985) 4702, Seite 963-967
  • Language: English
  • DOI: 10.1126/science.3890181
  • ISSN: 0036-8075; 1095-9203
  • Origination:
  • Footnote:
  • Description: The in vitro splicing reactions of pre-messenger RNA (pre-mRNA) in a yeast extract were analyzed by glycerol gradient centrifugation. Labeled pre-mRNA appears in a 40 S peak only if the pre-mRNA undergoes the first of the two partial splicing reactions. RNA analysis after extraction of glycerol gradient fractions shows that lariat-form intermediates, molecules that occur only in mRNA splicing, are found almost exclusively in this 40 S complex. Another reaction intermediate, cut 5′ exon RNA, can also be found concentrated in this complex. The complex is stable even in 400 m M KCl, although at this salt concentration, it sediments at 35 S and is clearly distinguishable from 40 S ribosomal subunits. This complex, termed a "spliceosome," is thought to contain components necessary for mRNA splicing; its existence can explain how separated exons on pre-mRNA are brought into contact.