• Media type: E-Article
  • Title: Expression of Treponema pallidum Antigens in Escherichia coli
  • Contributor: Walfield, Alan M.; Hanff, Philip A.; Lovett, Michael A.
  • imprint: American Association for the Advancement of Science (AAAS), 1982
  • Published in: Science
  • Language: English
  • DOI: 10.1126/science.7041257
  • ISSN: 0036-8075; 1095-9203
  • Keywords: Multidisciplinary
  • Origination:
  • Footnote:
  • Description: <jats:p> <jats:italic>Treponema pallidum</jats:italic> DNA was cloned in a bacteriophage. Clones were screened for expression of <jats:italic>Treponema pallidum</jats:italic> antigens by an in situ radioimmunoassay on nitrocellulose, with the use of subsequent reactions with syphilitic serum and radioiodinated <jats:italic>Staphylococcus aureus</jats:italic> protein A. One clone, which gave a strong signal, codes for at least seven antigens that react specifically with human antibodies to <jats:italic>Treponema pallidum</jats:italic> . </jats:p>