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Media type:
E-Article
Title:
Sister chromatid cohesion is mediated by individual cohesin complexes
Contributor:
Ochs, Fena;
Green, Charlotte;
Szczurek, Aleksander Tomasz;
Pytowski, Lior;
Kolesnikova, Sofia;
Brown, Jill;
Gerlich, Daniel Wolfram;
Buckle, Veronica;
Schermelleh, Lothar;
Nasmyth, Kim Ashley
Published:
American Association for the Advancement of Science (AAAS), 2024
Published in:
Science, 383 (2024) 6687, Seite 1122-1130
Language:
English
DOI:
10.1126/science.adl4606
ISSN:
0036-8075;
1095-9203
Origination:
Footnote:
Description:
Eukaryotic genomes are organized by loop extrusion and sister chromatid cohesion, both mediated by the multimeric cohesin protein complex. Understanding how cohesin holds sister DNAs together, and how loss of cohesion causes age-related infertility in females, requires knowledge as to cohesin’s stoichiometry in vivo. Using quantitative super-resolution imaging, we identified two discrete populations of chromatin-bound cohesin in postreplicative human cells. Whereas most complexes appear dimeric, cohesin that localized to sites of sister chromatid cohesion and associated with sororin was exclusively monomeric. The monomeric stoichiometry of sororin:cohesin complexes demonstrates that sister chromatid cohesion is conferred by individual cohesin rings, a key prediction of the proposal that cohesion arises from the co-entrapment of sister DNAs.