Severin, Anatoly;
Wu, Shang Wei;
Tabei, Keiko;
Tomasz, Alexander
Penicillin-Binding Protein 2 Is Essential for Expression of High-Level Vancomycin Resistance and Cell Wall Synthesis in Vancomycin- Resistant Staphylococcus aureus Carrying the Enterococcal vanA Gene Complex
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Media type:
E-Article
Title:
Penicillin-Binding Protein 2 Is Essential for Expression of High-Level Vancomycin Resistance and Cell Wall Synthesis in Vancomycin- Resistant Staphylococcus aureus Carrying the Enterococcal vanA Gene Complex
Contributor:
Severin, Anatoly;
Wu, Shang Wei;
Tabei, Keiko;
Tomasz, Alexander
Published:
American Society for Microbiology, 2004
Published in:
Antimicrobial Agents and Chemotherapy, 48 (2004) 12, Seite 4566-4573
Description:
<jats:title>ABSTRACT</jats:title>
<jats:p>
A combination of biochemical and genetic experiments were performed in order to better understand the mechanism of expression of high-level vancomycin resistance in
<jats:italic>Staphylococcus aureus</jats:italic>
. The transcription of
<jats:italic>pbp2</jats:italic>
of the highly vancomycin- and oxacillin-resistant strain COLVA
<jats:sub>200</jats:sub>
and its mutant derivative with inactivated
<jats:italic>mecA</jats:italic>
were put under the control of an inducible promoter, and the dependence of oxacillin and vancomycin resistance and cell wall composition on the concentration of the isopropyl-β-
<jats:sc>d</jats:sc>
-thiogalactopyranoside inducer was determined. The results indicate that
<jats:italic>mecA—</jats:italic>
the genetic determinant of oxacillin resistance—while essential for oxacillin resistance, is not involved with the expression of vancomycin resistance. Penicillin binding protein 2A, the protein product of
<jats:italic>mecA</jats:italic>
, appears to be unable to utilize the depsipeptide cell wall precursor produced in the vancomycin-resistant cells for transpeptidation. The key penicillin binding protein essential for vancomycin resistance and for the synthesis of the abnormally structured cell walls characteristic of vancomycin-resistant
<jats:italic>S. aureus</jats:italic>
(A. Severin, K. Tabei, F. Tenover, M. Chung, N. Clarke, and A. Tomasz, J. Biol. Chem.
<jats:italic>279</jats:italic>
:3398-3407, 2004) is penicillin binding protein 2.
</jats:p>