Nieto-Domínguez, Manuel;
de Eugenio, Laura I.;
Barriuso, Jorge;
Prieto, Alicia;
Fernández de Toro, Beatriz;
Canales-Mayordomo, Ángeles;
Martínez, María Jesús
Novel pH-Stable Glycoside Hydrolase Family 3 β-Xylosidase from Talaromyces amestolkiae: an Enzyme Displaying Regioselective Transxylosylation
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Media type:
E-Article
Title:
Novel pH-Stable Glycoside Hydrolase Family 3 β-Xylosidase from Talaromyces amestolkiae: an Enzyme Displaying Regioselective Transxylosylation
Contributor:
Nieto-Domínguez, Manuel;
de Eugenio, Laura I.;
Barriuso, Jorge;
Prieto, Alicia;
Fernández de Toro, Beatriz;
Canales-Mayordomo, Ángeles;
Martínez, María Jesús
Published:
American Society for Microbiology, 2015
Published in:
Applied and Environmental Microbiology, 81 (2015) 18, Seite 6380-6392
Language:
English
DOI:
10.1128/aem.01744-15
ISSN:
0099-2240;
1098-5336
Origination:
Footnote:
Description:
ABSTRACT This paper reports on a novel β-xylosidase from the hemicellulolytic fungus Talaromyces amestolkiae . The expression of this enzyme, called BxTW1, could be induced by beechwood xylan and was purified as a glycoprotein from culture supernatants. We characterized the gene encoding this enzyme as an intronless gene belonging to the glycoside hydrolase gene family 3 (GH3). BxTW1 exhibited transxylosylation activity in a regioselective way. This feature would allow the synthesis of oligosaccharides or other compounds not available from natural sources, such as alkyl glycosides displaying antimicrobial or surfactant properties. Regioselective transxylosylation, an uncommon combination, makes the synthesis reproducible, which is desirable for its potential industrial application. BxTW1 showed high pH stability and Cu 2+ tolerance. The enzyme displayed a pI of 7.6, a molecular mass around 200 kDa in its active dimeric form, and K m and V max values of 0.17 mM and 52.0 U/mg, respectively, using commercial p -nitrophenyl-β- d -xylopyranoside as the substrate. The catalytic efficiencies for the hydrolysis of xylooligosaccharides were remarkably high, making it suitable for different applications in food and bioenergy industries.