• Media type: E-Article
  • Title: A Molecular Biology Tool Kit for the Phototrophic Firmicute Heliobacterium modesticaldum
  • Contributor: Baker, Patricia L.; Orf, Gregory S.; Khan, Zahid; Espinoza, Levi; Leung, Sabrina; Kevershan, Kimberly; Redding, Kevin E.
  • imprint: American Society for Microbiology, 2019
  • Published in: Applied and Environmental Microbiology
  • Language: English
  • DOI: 10.1128/aem.01287-19
  • ISSN: 0099-2240; 1098-5336
  • Keywords: Ecology ; Applied Microbiology and Biotechnology ; Food Science ; Biotechnology
  • Origination:
  • Footnote:
  • Description: <jats:p>Heliobacteria are anoxygenic phototrophic bacteria with the simplest known photosynthetic apparatus. They are unique in using bacteriochlorophyll<jats:italic>g</jats:italic>as their main pigment and lacking a peripheral antenna system. Until now, research on this organism has been hampered by the lack of a genetic transformation system. Without such a system, gene knockouts, site-directed mutations, and gene expression studies cannot be performed to help us further understand or manipulate the organism. Here we report the genetic transformation of a heliobacterium, which should enable future genetic studies in this unique phototrophic organism.</jats:p>
  • Access State: Open Access