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Media type:
E-Article
Title:
Production of Glutaconic Acid in a RecombinantEscherichia coliStrain
Contributor:
Djurdjevic, Ivana;
Zelder, Oskar;
Buckel, Wolfgang
Published:
American Society for Microbiology, 2011
Published in:
Applied and Environmental Microbiology, 77 (2011) 1, Seite 320-322
Language:
English
DOI:
10.1128/aem.02049-10
ISSN:
0099-2240;
1098-5336
Origination:
Footnote:
Description:
ABSTRACTThe assembly of six genes that encode enzymes from glutamate-fermenting bacteria convertedEscherichia coliinto a glutaconate producer when grown anaerobically on a complex medium. The new anaerobic pathway starts with 2-oxoglutarate from general metabolism and proceeds via (R)-2-hydroxyglutarate, (R)-2-hydroxyglutaryl-coenzyme A (CoA), and (E)-glutaconyl-CoA to yield 2.7 ± 0.2 mM (E)-glutaconate in the medium.