Fritsch, Isabel;
Luyven, Gabriele;
Köhler, Heike;
Lutz, Walburga;
Möbius, Petra
Suspicion of Mycobacterium avium subsp. paratuberculosis Transmission between Cattle and Wild-Living Red Deer (Cervus elaphus) by Multitarget Genotyping
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Media type:
E-Article
Title:
Suspicion of Mycobacterium avium subsp. paratuberculosis Transmission between Cattle and Wild-Living Red Deer (Cervus elaphus) by Multitarget Genotyping
Description:
<jats:title>ABSTRACT</jats:title>
<jats:p>
Multitarget genotyping of the etiologic agent
<jats:named-content content-type="genus-species">Mycobacterium avium</jats:named-content>
subsp.
<jats:named-content content-type="genus-species">paratuberculosis</jats:named-content>
is necessary for epidemiological tracing of paratuberculosis (Johne's disease). The study was undertaken to assess the informative value of different typing techniques and individual genome markers by investigation of
<jats:named-content content-type="genus-species">M. avium</jats:named-content>
subsp.
<jats:named-content content-type="genus-species">paratuberculosis</jats:named-content>
transmission between wild-living red deer and farmed cattle with known shared habitats. Fifty-three
<jats:named-content content-type="genus-species">M. avium</jats:named-content>
subsp.
<jats:named-content content-type="genus-species">paratuberculosis</jats:named-content>
type II isolates were differentiated by short sequence repeat analysis (SSR; 4 loci), mycobacterial interspersed repetitive-unit–variable-number tandem-repeat analysis (MIRU-VNTR; 8 loci), and restriction fragment length polymorphism analysis based on IS
<jats:italic>900</jats:italic>
(IS
<jats:italic>900</jats:italic>
-RFLP) using BstEII and PstI digestion. Isolates originated from free-living red deer (
<jats:named-content content-type="genus-species">Cervus elaphus</jats:named-content>
) from Eifel National Park (
<jats:italic>n</jats:italic>
= 13), six cattle herds living in the area of this park (
<jats:italic>n</jats:italic>
= 23), and five cattle herds without any contact with these red deer (
<jats:italic>n</jats:italic>
= 17). Data based on individual herds and genotypes verified that SSR G2 repeats did not exhibit sufficient stability for epidemiological studies. Two common SSR profiles (without G2 repeats), nine MIRU-VNTR patterns, and nine IS
<jats:italic>900</jats:italic>
-RFLP patterns were detected, resulting in 17 genotypes when combined. A high genetic variability was found for red deer and cattle isolates within and outside Eifel National Park, but it was revealed only by combination of different typing techniques. Results imply that within this restricted area, wild-living and farmed animals maintain a reservoir for specific
<jats:named-content content-type="genus-species">M. avium</jats:named-content>
subsp.
<jats:named-content content-type="genus-species">paratuberculosis</jats:named-content>
genotypes. No host relation of genotypes was obtained. Results suggested that four genotypes had been transmitted between and within species and that one genotype had been transmitted between cattle herds only. Use of multitarget genotyping for
<jats:named-content content-type="genus-species">M. avium</jats:named-content>
subsp.
<jats:named-content content-type="genus-species">paratuberculosis</jats:named-content>
type II strains and sufficiently stable genetic markers is essential for reliable interpretations of epidemiological studies on paratuberculosis.
</jats:p>