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Media type:
E-Article
Title:
Campylobacter jejuniColonization of Mice with Limited Enteric Flora
Contributor:
Chang, Christopher;
Miller, Jeff F.
imprint:
American Society for Microbiology, 2006
Published in:Infection and Immunity
Language:
English
DOI:
10.1128/iai.01094-05
ISSN:
0019-9567;
1098-5522
Origination:
Footnote:
Description:
<jats:title>ABSTRACT</jats:title><jats:p>We have developed experimental murine<jats:italic>Campylobacter</jats:italic>infection models which demonstrate efficient establishment and reproducible, high-level colonization. Following oral inoculation, wild-type C3H mice with normal enteric flora were colonized inconsistently and inefficiently by<jats:italic>C. jejuni</jats:italic>strain 81-176. However, C3H mice with a limited gut flora (LF) were efficiently colonized at high levels (10<jats:sup>8</jats:sup>CFU/g of stool or large intestine tissue) followed by clearance after several weeks. Large intestine tissue showed minimal to mild inflammation at days 7 and 28 postinoculation. In striking contrast, C3H SCID mice with the same limited flora remained persistently colonized at a consistently high level until they were euthanized 8 months postinoculation. Lower gastrointestinal tract tissue from LF-SCID mice showed marked to severe inflammation in the colon and cecum at days 7 and 28 and intense inflammation of the stomach at day 28. These findings indicate that although the innate response alone cannot block colonization persistence, it is sufficient to orchestrate marked gut inflammation. Moreover, the adaptive immune response is critical to mediate<jats:italic>C. jejuni</jats:italic>clearance from the colonized gut. To validate our LF murine model, we verified that motility and chemotaxis are critical for colonization. Insertion-deletion mutations were generated in<jats:italic>motB</jats:italic>and<jats:italic>fliI</jats:italic>, which encode products essential for motility and flagellar assembly, and in the presumptive chemotaxis gene<jats:italic>cheA</jats:italic>(histidine kinase). All mutants failed to establish colonization in LF mice. Our limited flora murine colonization models serve as tractable, reproducible tools to define host responses to<jats:italic>C. jejuni</jats:italic>infection and to identify and characterize virulence determinants required for colonization.</jats:p>