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Media type:
E-Article
Title:
Essential Role of the Type III Secretion System Effector NleB in Colonization of Mice by Citrobacter rodentium
Contributor:
Kelly, Michelle;
Hart, Emily;
Mundy, Rosanna;
Marchès, Olivier;
Wiles, Siouxsie;
Badea, Luminita;
Luck, Shelley;
Tauschek, Marija;
Frankel, Gad;
Robins-Browne, Roy M.;
Hartland, Elizabeth L.
imprint:
American Society for Microbiology, 2006
Published in:Infection and Immunity
Language:
English
DOI:
10.1128/iai.74.4.2328-2337.2006
ISSN:
0019-9567;
1098-5522
Origination:
Footnote:
Description:
<jats:title>ABSTRACT</jats:title>
<jats:p>
Attaching and effacing (A/E) pathogens are a significant cause of gastrointestinal illness in humans and animals. All A/E pathogens carry a large pathogenicity island, termed the locus for enterocyte effacement (LEE), which encodes a type III secretion system that translocates several effector proteins into host cells. To identify novel virulence determinants in A/E pathogens, we performed a signature-tagged mutagenesis screen in C57BL/6 mice by using the mouse A/E pathogen
<jats:italic>Citrobacter rodentium</jats:italic>
. Five hundred seventy-six derivatives of
<jats:italic>C. rodentium</jats:italic>
were tested in pools of 12 mutants. One attenuated mutant carried a transposon insertion in
<jats:italic>nleB</jats:italic>
, which encodes a putative effector of the LEE-encoded type III secretion system (T3SS).
<jats:italic>nleB</jats:italic>
is present in a genomic pathogenicity island that also encodes another putative effector, NleE, immediately downstream. Using translational fusions with β-lactamase (TEM-1), we showed that both NleB and NleE were translocated into host cells by the LEE-encoded T3SS of enteropathogenic
<jats:italic>Escherichia coli</jats:italic>
. In addition, deletion of the gene encoding NleB in
<jats:italic>C. rodentium</jats:italic>
resulted in reduced colonization of mice in single infections and reduced colonic hyperplasia. In contrast, the deletion of other non-LEE-encoded effector genes in
<jats:italic>C. rodentium</jats:italic>
,
<jats:italic>nleC</jats:italic>
,
<jats:italic>nleD</jats:italic>
, or
<jats:italic>nleE</jats:italic>
, had no effect on host colonization or disease. These results suggest that
<jats:italic>nleB</jats:italic>
encodes an important virulence determinant of A/E pathogens.
</jats:p>