Description:
<jats:p>
A mutation that causes a temperature-sensitive RecA
<jats:sup>−</jats:sup>
phenotype was identified in a derivative of a PolA
<jats:sup>−</jats:sup>
strain that failed to grow at high temperature. The mutant allele (
<jats:italic>recA200</jats:italic>
) was shown to be linked to
<jats:italic>cysC</jats:italic>
, conferred a sharply temperature-sensitive, ultraviolet-sensitive Rec
<jats:sup>−</jats:sup>
phenotype in the range 35 to 42 C, and in crosses failed to show complementation at 42 C with Hfr's that transferred
<jats:italic>recA</jats:italic>
<jats:sup>−</jats:sup>
. Double mutants that carried both
<jats:italic>recA200</jats:italic>
and
<jats:italic>polA</jats:italic>
were examined for ability to grow and synthesize DNA at restrictive temperatures.
</jats:p>