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Media type:
E-Article
Title:
The Genes Encoding Formamidopyrimidine and MutY DNA Glycosylases in Escherichia coli Are Transcribed as Part of Complex Operons
Contributor:
Gifford, Christine M.;
Wallace, Susan S.
imprint:
American Society for Microbiology, 1999
Published in:Journal of Bacteriology
Language:
English
DOI:
10.1128/jb.181.14.4223-4236.1999
ISSN:
0021-9193;
1098-5530
Origination:
Footnote:
Description:
<jats:title>ABSTRACT</jats:title>
<jats:p>
<jats:italic>Escherichia coli</jats:italic>
formamidopyrimidine (Fpg) DNA glycosylase and MutY DNA glycosylase are base excision repair proteins that work together to protect cells from the mutagenic effects of the commonly oxidized guanine product 7,8-dihydro-8-oxoguanine. The genes encoding these proteins,
<jats:italic>fpg</jats:italic>
and
<jats:italic>mutY</jats:italic>
, are both cotranscribed as part of complex operons.
<jats:italic>fpg</jats:italic>
is the terminal gene in an operon with the gene order
<jats:italic>radC</jats:italic>
,
<jats:italic>rpmB</jats:italic>
,
<jats:italic>rpmG</jats:italic>
, and
<jats:italic>fpg</jats:italic>
. This operon has transcription initiation sites upstream of
<jats:italic>radC</jats:italic>
, in the
<jats:italic>radC</jats:italic>
coding region, and immediately upstream of
<jats:italic>fpg</jats:italic>
. There is a strong attenuator in the
<jats:italic>rpmG-fpg</jats:italic>
intergenic region and three transcription termination sites downstream of
<jats:italic>fpg</jats:italic>
. There is an additional site, in the
<jats:italic>radC-rpmB</jats:italic>
intergenic region, that corresponds either to a transcription initiation site or to an RNase E or RNase III cleavage site.
<jats:italic>mutY</jats:italic>
is the first gene in an operon with the gene order
<jats:italic>mutY</jats:italic>
,
<jats:italic>yggX</jats:italic>
,
<jats:italic>mltC</jats:italic>
, and
<jats:italic>nupG</jats:italic>
. This operon has transcription initiation sites upstream of
<jats:italic>mutY</jats:italic>
, in the
<jats:italic>mutY</jats:italic>
coding region, and immediately upstream of
<jats:italic>nupG</jats:italic>
. There also appear to be attenuators in the
<jats:italic>yggX-mltC</jats:italic>
and
<jats:italic>mltC-nupG</jats:italic>
intergenic regions. The order of genes in these operons has been conserved or partially conserved only in other closely related gram-negative bacteria, although it is not known whether the genes are cotranscribed in these other organisms.
</jats:p>