Wilde, Caroline;
Bachellier, Sophie;
Hofnung, Maurice;
Clément, Jean-Marie
Transposition of IS 1397 in the Family Enterobacteriaceae and First Characterization of ISKpn 1 , a New Insertion Sequence Associated with Klebsiella pneumoniae Palindromic Units
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Media type:
E-Article
Title:
Transposition of IS 1397 in the Family Enterobacteriaceae and First Characterization of ISKpn 1 , a New Insertion Sequence Associated with Klebsiella pneumoniae Palindromic Units
Contributor:
Wilde, Caroline;
Bachellier, Sophie;
Hofnung, Maurice;
Clément, Jean-Marie
Published:
American Society for Microbiology, 2001
Published in:
Journal of Bacteriology, 183 (2001) 15, Seite 4395-4404
Language:
English
DOI:
10.1128/jb.183.15.4395-4404.2001
ISSN:
0021-9193;
1098-5530
Origination:
Footnote:
Description:
ABSTRACT IS 1397 and ISKpn 1 are IS 3 family members which are specifically inserted into the loop of palindromic units (PUs). IS 1397 is shown to transpose into PUs with sequences close or identical to the Escherichia coli consensus, even in other enterobacteria ( Salmonella enterica serovar Typhimurium, Klebsiella pneumoniae , and Klebsiella oxytoca ). Moreover, we show that homologous intergenic regions containing PUs constitute IS 1397 transpositional hot spots, despite bacterial interspersed mosaic element structures that differ among the three species. ISKpn 1 , described here for the first time, is specific for PUs from K. pneumoniae , in which we discovered it. A sequence comparison between the two insertion sequences allowed us to define a motif possibly accounting for their specificity.