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Media type:
E-Article
Title:
Nucleocapsid Protein Recruitment to Replication-Transcription Complexes Plays a Crucial Role in Coronaviral Life Cycle
Contributor:
Cong, Yingying;
Ulasli, Mustafa;
Schepers, Hein;
Mauthe, Mario;
V’kovski, Philip;
Kriegenburg, Franziska;
Thiel, Volker;
de Haan, Cornelis A. M.;
Reggiori, Fulvio
imprint:
American Society for Microbiology, 2020
Published in:Journal of Virology
Language:
English
DOI:
10.1128/jvi.01925-19
ISSN:
0022-538X;
1098-5514
Origination:
Footnote:
Description:
<jats:p>CoVs have long been regarded as relatively harmless pathogens for humans. Severe respiratory tract infection outbreaks caused by severe acute respiratory syndrome CoV and Middle East respiratory syndrome CoV, however, have caused high pathogenicity and mortality rates in humans. These outbreaks highlighted the relevance of being able to control CoV infections. We used a model CoV, MHV, to investigate the importance of the recruitment of N protein, a central component of CoV virions, to intracellular platforms where CoVs replicate, transcribe, and translate their genomes. By identifying the principal binding partner at these intracellular platforms and generating a specific mutant, we found that N protein recruitment to these locations is crucial for promoting viral RNA synthesis. Moreover, blocking this recruitment strongly inhibits viral infection. Thus, our results explain an important aspect of the CoV life cycle and reveal an interaction of viral proteins that could be targeted in antiviral therapies.</jats:p>