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Media type:
E-Article
Title:
Tissue-Resident Macrophages Are Productively Infected Ex Vivo by Primary X4 Isolates of Human Immunodeficiency Virus Type 1
Contributor:
Jayakumar, Prerana;
Berger, Irina;
Autschbach, Frank;
Weinstein, Mark;
Funke, Benjamin;
Verdin, Eric;
Goldsmith, Mark A.;
Keppler, Oliver T.
Published:
American Society for Microbiology, 2005
Published in:
Journal of Virology, 79 (2005) 8, Seite 5220-5226
Language:
English
DOI:
10.1128/jvi.79.8.5220-5226.2005
ISSN:
0022-538X;
1098-5514
Origination:
Footnote:
Description:
<jats:title>ABSTRACT</jats:title><jats:p>Infection of macrophages has been implicated as a critical event in the transmission and persistence of human immunodeficiency virus type 1 (HIV-1). Here, we explore whether primary X4 HIV-1 isolates can productively infect tissue macrophages that have terminally differentiated in vivo. Using immunohistochemistry, HIV-1 RNA in situ hybridization, and confocal immunofluorescence microscopy, we demonstrate that macrophages residing in human tonsil blocks can be productively infected ex vivo by primary X4 HIV-1 isolates. This challenges the model in which macrophage tropism is a key determinant of the selective transmission of R5 HIV-1 strains. Infection of tissue macrophages by X4 HIV-1 may be highly relevant in vivo and contribute to key events in HIV-1 pathogenesis.</jats:p>