> Details

Benitz, Simone; Straub, Tobias; Mahajan, Ujjwal Mukund; Mutter, Jurik; Czemmel, Stefan; Unruh, Tatjana; Wingerath, Britta; Deubler, Sabrina; Fahr, Lisa; Cheng, Tao; Nahnsen, Sven; Bruns, Philipp; Kong, Bo; Raulefs, Susanne; Ceyhan, Güralp O; Mayerle, Julia; Steiger, Katja; Esposito, Irene; Kleeff, Jörg; Michalski, Christoph W; Regel, Ivonne

Ring1b-dependent epigenetic remodelling is an essential prerequisite for pancreatic carcinogenesis

Reference
management

Bookmarks

Remove from
bookmarks

Share this on Whatsapp

Close

Bookmarks



You can manage bookmarks using lists, please log in to your user account for this.
  • Media type: E-Article
  • Title: Ring1b-dependent epigenetic remodelling is an essential prerequisite for pancreatic carcinogenesis
  • Contributor: Benitz, Simone; Straub, Tobias; Mahajan, Ujjwal Mukund; Mutter, Jurik; Czemmel, Stefan; Unruh, Tatjana; Wingerath, Britta; Deubler, Sabrina; Fahr, Lisa; Cheng, Tao; Nahnsen, Sven; Bruns, Philipp; Kong, Bo; Raulefs, Susanne; Ceyhan, Güralp O; Mayerle, Julia; Steiger, Katja; Esposito, Irene; Kleeff, Jörg; Michalski, Christoph W; Regel, Ivonne
  • Published: BMJ, 2019
  • Published in: Gut, 68 (2019) 11, Seite 2007-2018
  • Language: English
  • DOI: 10.1136/gutjnl-2018-317208
  • ISSN: 0017-5749; 1468-3288
  • Origination:
  • Footnote:
  • Description: Background and aimsBesides well-defined genetic alterations, the dedifferentiation of mature acinar cells is an important prerequisite for pancreatic carcinogenesis. Acinar-specific genes controlling cell homeostasis are extensively downregulated during cancer development; however, the underlying mechanisms are poorly understood. Now, we devised a novel in vitro strategy to determine genome-wide dynamics in the epigenetic landscape in pancreatic carcinogenesis.DesignWith our in vitro carcinogenic sequence, we performed global gene expression analysis and ChIP sequencing for the histone modifications H3K4me3, H3K27me3 and H2AK119ub. Followed by a comprehensive bioinformatic approach, we captured gene clusters with extensive epigenetic and transcriptional remodelling. Relevance of Ring1b-catalysed H2AK119ub in acinar cell reprogramming was studied in an inducible Ring1b knockout mouse model. CRISPR/Cas9-mediated Ring1b ablation as well as drug-induced Ring1b inhibition were functionally characterised in pancreatic cancer cells.ResultsThe epigenome is vigorously modified during pancreatic carcinogenesis, defining cellular identity. Particularly, regulatory acinar cell transcription factors are epigenetically silenced by the Ring1b-catalysed histone modification H2AK119ub in acinar-to-ductal metaplasia and pancreatic cancer cells. Ring1b knockout mice showed greatly impaired acinar cell dedifferentiation and pancreatic tumour formation due to a retained expression of acinar differentiation genes. Depletion or drug-induced inhibition of Ring1b promoted tumour cell reprogramming towards a less aggressive phenotype.ConclusionsOur data provide substantial evidence that the epigenetic silencing of acinar cell fate genes is a mandatory event in the development and progression of pancreatic cancer. Targeting the epigenetic repressor Ring1b could offer new therapeutic options.