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Raila, Jens; Kalk, Philipp; Pfab, Thiemo; Thöne-Reineke, Christa; Godes, Michael; Yanagisawa, Masashi; Schweigert, Florian J.; Hocher, Berthold

Urinary protein profiling with surface-enhanced laser desorption/ionization time-of-flight mass spectrometry in ETBreceptor-deficient ratsThis article is one of a selection of papers published in the special issue (part 2 of 2) on Forefronts in Endothelin

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  • Media type: E-Article
  • Title: Urinary protein profiling with surface-enhanced laser desorption/ionization time-of-flight mass spectrometry in ETBreceptor-deficient ratsThis article is one of a selection of papers published in the special issue (part 2 of 2) on Forefronts in Endothelin
  • Contributor: Raila, Jens; Kalk, Philipp; Pfab, Thiemo; Thöne-Reineke, Christa; Godes, Michael; Yanagisawa, Masashi; Schweigert, Florian J.; Hocher, Berthold
  • imprint: Canadian Science Publishing, 2008
  • Published in: Canadian Journal of Physiology and Pharmacology
  • Language: English
  • DOI: 10.1139/y08-056
  • ISSN: 0008-4212; 1205-7541
  • Keywords: Physiology (medical) ; Pharmacology ; General Medicine ; Physiology
  • Origination:
  • Footnote:
  • Description: <jats:p>The pathways leading to salt-sensitive hypertension and renal damage in rescued ET<jats:sub>B</jats:sub>receptor-deficient (ETBRd) rats are still unknown. The objective of the study was therefore to identify modifications of urinary peptide and protein expression in ETBRd rats (n = 9) and wild-type controls (n = 6) using SDS – polyacrylamide gel electrophoresis (SDS-PAGE) and surface-enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI-TOF-MS) technology. Glomerular filtration rate, glomerulosclerosis, and tubulointerstitial fibrosis did not differ between the groups. ETBRd rats showed slightly higher blood pressure (p &lt; 0.001), media/lumen ratio of intrarenal arteries (p &lt; 0.01), and albuminuria (p &lt; 0.01). SDS-PAGE confirmed albuminuria, but showed no differences in the urinary excretion of low molecular weight proteins (&lt;60 kDa). SELDI-TOF-MS profiling revealed 9 proteomic features at molecular masses (Da) of 2720, 2980, 3130, 3345, 6466, 6682, 8550, 18 729, and 37 492, which were significantly elevated (p &lt; 0.02) in urine of ETBRd rats. The results demonstrate that, independent of structural changes in the kidneys, ET<jats:sub>B</jats:sub>-receptor deficiency causes specific differences in urinary peptide and protein excretion. SELDI-TOF-MS may be a valuable tool for the characterization of urinary biomarkers helping to uncover the mechanism of ETBR action in the kidney.</jats:p>