You can manage bookmarks using lists, please log in to your user account for this.
Media type:
E-Article
Title:
Lipid droplet formation in response to oleic acid in Huh-7 cells is a fatty acid receptor mediated event
Contributor:
Rohwedder, Arndt;
Zhang, Qifeng;
Rudge, Simon A.;
Wakelam, Michael J. O.
Published:
The Company of Biologists, 2014
Published in:
Journal of Cell Science (2014)
Language:
English
DOI:
10.1242/jcs.145854
ISSN:
1477-9137;
0021-9533
Origination:
Footnote:
Description:
It is unclear how changes in lipid droplet size and number are regulated, whether this involves a signalling pathway, or is directed by cellular lipid uptake. Here we show that oleic acid stimulates lipid droplet formation through activating the long chain fatty acid receptor FFAR4 coupled through a pertussis toxin sensitive G-protein signalling pathway involving phosphoinositide-3-kinase, AKT/Protein-kinase B and phospholipase D activities. This initial lipid droplet formation is not dependent upon exogenous lipid whilst the subsequent, more sustained increase in lipid droplets is dependent upon lipid uptake. These two mechanisms of lipid droplet formation point to distinct potential intervention points.