Description:
<jats:title>SUMMARY</jats:title>
<jats:p>To characterize the effect of severe hypoxia on neuronal activity,long-term intracellular recordings were made from neurones in the isolated central ring ganglia of Lymnaea stagnalis. When a neurone at rest in normoxia was subjected to severe hypoxia, action potential firing frequency decreased by 38% (from 2.4-1.5 spikes s-1), and the resting membrane potential hyperpolarized from -70.3 to -75.1 mV. Blocking GABAA receptor-mediated synaptic transmission with the antagonist bicuculline methiodide (100 μmol l-1) decreased neuronal activity by 36%, and prevented any further changes in response to severe hypoxia, indicating that GABAergic neurotransmission mediates the severe hypoxia-induced decrease in neuronal activity. Puffing 100 μmol l-1 GABA onto the cell body produced an excitatory response characterized by a transient increase in action potential (AP) firing, which was significantly decreased in severe hypoxia. Perturbing intracellular chloride concentrations with the Na+/K+/Cl-(NKCC1) cotransporter antagonist bumetanide (100 μmol l-1)decreased AP firing by 40%, consistent with GABA being an excitatory neurotransmitter in the adult Lymnaea CNS. Taken together, these studies indicate that severe hypoxia reduces the activity of NKCC1, leading to a reduction in excitatory GABAergic transmission, which results in a hyperpolarization of the resting membrane potential (Vm)and as a result decreased AP frequency.</jats:p>